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1.
Developmental Plasticity at High Temperature.
Vu, LD, Xu, X, Gevaert, K, De Smet, I
Plant physiology. 2019;(2):399-411
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Abstract
Molecular mechanisms controlling the thermal response in Arabidopsis.
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2.
Arabidopsis acyl-CoA-binding proteins regulate the synthesis of lipid signals.
Lung, SC, Chye, ML
The New phytologist. 2019;(1):113-117
Abstract
Plant lipid signals are crucial developmental modulators and stress response mediators. A family of acyl-CoA-binding proteins (ACBPs) participates in the lipid trafficking of these signals. Isoform-specific functions can arise from differences in their subcellular distribution, tissue-specificity, stress-responsiveness, and ligand selectivity. In lipid-mediated cell signaling, plant ACBPs are not merely transporters but are also important regulators via their interaction with lipid-metabolic enzymes and precursor lipids. In this Insight, the regulatory roles of plant ACBPs in the synthesis of various signaling lipids, including phosphatidic acid, sterols, oxylipins, and sphingolipids, are reviewed. We focus on the functional significance of these lipid signals in plant development and stress responses with an overview of recent work using reverse genetics and transgenic Arabidopsis.
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3.
Translating auxin responses into ovules, seeds and yield: Insight from Arabidopsis and the cereals.
Shirley, NJ, Aubert, MK, Wilkinson, LG, Bird, DC, Lora, J, Yang, X, Tucker, MR
Journal of integrative plant biology. 2019;(3):310-336
Abstract
Grain production in cereal crops depends on the stable formation of male and female gametes in the flower. In most angiosperms, the female gamete is produced from a germline located deep within the ovary, protected by several layers of maternal tissue, including the ovary wall, ovule integuments and nucellus. In the field, germline formation and floret fertility are major determinants of yield potential, contributing to traits such as seed number, weight and size. As such, stimuli affecting the timing and duration of reproductive phases, as well as the viability, size and number of cells within reproductive organs can significantly impact yield. One key stimulant is the phytohormone auxin, which influences growth and morphogenesis of female tissues during gynoecium development, gametophyte formation, and endosperm cellularization. In this review we consider the role of the auxin signaling pathway during ovule and seed development, first in the context of Arabidopsis and then in the cereals. We summarize the gene families involved and highlight distinct expression patterns that suggest a range of roles in reproductive cell specification and fate. This is discussed in terms of seed production and how targeted modification of different tissues might facilitate improvements.
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ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense.
Rustgi, S, Springer, A, Kang, C, von Wettstein, D, Reinbothe, C, Reinbothe, S, Pollmann, S
International journal of molecular sciences. 2019;(12)
Abstract
The channeling of metabolites is an essential step of metabolic regulation in all living organisms. Multifunctional enzymes with defined domains for metabolite compartmentalization are rare, but in many cases, larger assemblies forming multimeric protein complexes operate in defined metabolic shunts. In Arabidopsis thaliana, a multimeric complex was discovered that contains a 13-lipoxygenase and allene oxide synthase (AOS) as well as allene oxide cyclase. All three plant enzymes are localized in chloroplasts, contributing to the biosynthesis of jasmonic acid (JA). JA and its derivatives act as ubiquitous plant defense regulators in responses to both biotic and abiotic stresses. AOS belongs to the superfamily of cytochrome P450 enzymes and is named CYP74A. Another CYP450 in chloroplasts, hydroperoxide lyase (HPL, CYP74B), competes with AOS for the common substrate. The products of the HPL reaction are green leaf volatiles that are involved in the deterrence of insect pests. Both enzymes represent non-canonical CYP450 family members, as they do not depend on O2 and NADPH-dependent CYP450 reductase activities. AOS and HPL activities are crucial for plants to respond to different biotic foes. In this mini-review, we aim to summarize how plants make use of the LOX2-AOS-AOC2 complex in chloroplasts to boost JA biosynthesis over volatile production and how this situation may change in plant communities during mass ingestion by insect pests.
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5.
Mitochondrial complex II of plants: subunit composition, assembly, and function in respiration and signaling.
Huang, S, Braun, HP, Gawryluk, RMR, Millar, AH
The Plant journal : for cell and molecular biology. 2019;(3):405-417
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Abstract
Complex II [succinate dehydrogenase (succinate-ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5-SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1-SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1-SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species-dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate-dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility.
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6.
ABA Transport and Plant Water Stress Responses.
Kuromori, T, Seo, M, Shinozaki, K
Trends in plant science. 2018;(6):513-522
Abstract
To understand the integrative networks of signaling molecules, the sites of their biosynthesis and action must be clarified, particularly for phytohormones such as abscisic acid (ABA). The relationship between the sites of ABA biosynthesis and transport has been discussed extensively in the context of guard cells and stomatal regulation. However, guard cells are not the only site of ABA action. Recent studies have reported multiple sites of ABA biosynthesis and multiple ABA transporters, indicating that ABA transport regulation is not unidirectional but rather forms complex networks. Therefore, it is important to determine how multiple ABA sources coordinately contribute to individual biological processes under various physiological conditions.
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Bioinformatics Resources for Plant Genomics: Opportunities and Bottlenecks in The -omics Era.
Ambrosino, L, Colantuono, C, Monticolo, F, Chiusano, ML
Current issues in molecular biology. 2018;:71-88
Abstract
The sudden exponential increase of biological data concerning genome structure and functionalities, also fostered by the advent of Next Generation Sequencing (NGS) technologies, while expanding the opportunity to highlight still uncovered molecular aspects, challenges bioinformatics in several repects. Data management, processing, updating, dissemination and integration are the major areas of concern. The rapid increase in various omics technologies causes two major issues, which may even appear contrasting: the dissemination of poorly curated datasets, still in the form of raw collections or preliminary draft results, and the fast updating of information that, as a consequence, affects the establishment of stable reliable resources. These issues are mainly caused by the lower rate of bioinformatics in extracting added value information from the large amount of data, when compared to the faster technologies involved in data production. This review describes main bioinformatics resources for plants genomics to underline the heterogeneity of the available collections, coherent with the multifaceted complexity of plant sciences. It aims to provide an in-depth report highlighting bottlenecks that may significantly affect a fluent progress in the field and attempts to suggest possible solutions to the various issues.
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Mining and Development of Novel SSR Markers Using Next Generation Sequencing (NGS) Data in Plants.
Taheri, S, Lee Abdullah, T, Yusop, MR, Hanafi, MM, Sahebi, M, Azizi, P, Shamshiri, RR
Molecules (Basel, Switzerland). 2018;(2)
Abstract
Microsatellites, or simple sequence repeats (SSRs), are one of the most informative and multi-purpose genetic markers exploited in plant functional genomics. However, the discovery of SSRs and development using traditional methods are laborious, time-consuming, and costly. Recently, the availability of high-throughput sequencing technologies has enabled researchers to identify a substantial number of microsatellites at less cost and effort than traditional approaches. Illumina is a noteworthy transcriptome sequencing technology that is currently used in SSR marker development. Although 454 pyrosequencing datasets can be used for SSR development, this type of sequencing is no longer supported. This review aims to present an overview of the next generation sequencing, with a focus on the efficient use of de novo transcriptome sequencing (RNA-Seq) and related tools for mining and development of microsatellites in plants.
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Update on amino acid transporter functions and on possible amino acid sensing mechanisms in plants.
Dinkeloo, K, Boyd, S, Pilot, G
Seminars in cell & developmental biology. 2018;:105-113
Abstract
Amino acids are essential components of plant metabolism, not only as constituents of proteins, but also as precursors of important secondary metabolites and as carriers of organic nitrogen between the organs of the plant. Transport across intracellular membranes and translocation of amino acids within the plant is mediated by membrane amino acid transporters. The past few years have seen the identification of a new family of amino acid transporters in Arabidopsis, the characterization of intracellular amino acid transporters, and the discovery of new roles for already known proteins. While amino acid metabolism needs to be tightly coordinated with amino acid transport activity and carbohydrate metabolism, no gene involved in amino acid sensing in plants has been unequivocally identified to date. This review aims at summarizing the recent data accumulated on the identity and function of amino acid transporters in plants, and discussing the possible identity of amino acid sensors based on data from other organisms.
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10.
Breakout-lateral root emergence in Arabidopsis thaliana.
Stoeckle, D, Thellmann, M, Vermeer, JE
Current opinion in plant biology. 2018;:67-72
Abstract
Lateral roots are determinants of plant root system architecture. Besides providing anchorage, they are a plant's means to explore the soil environment for water and nutrients. Lateral roots form post-embryonically and initiate deep within the root. On its way to the surface, the newly formed organ needs to grow through three overlying cell layers; the endodermis, cortex and epidermis. A picture is emerging that a tight integration of chemical and mechanical signalling between the lateral root and the surrounding tissue is essential for proper organogenesis. Here we review the latest progress made towards our understanding of the fascinating biology underlying lateral root emergence in Arabidopsis.