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1.
Anhydrobiosis: Inside yeast cells.
Rapoport, A, Golovina, EA, Gervais, P, Dupont, S, Beney, L
Biotechnology advances. 2019;(1):51-67
Abstract
Under natural conditions yeast cells as well as other microorganisms are regularly subjected to the influence of severe drought, which leads to their serious dehydration. The dry seasons are then changed by rains and there is a restoration of normal water potential inside the cells. To survive such seasonal changes a lot of vegetative microbial cells, which belong to various genera and species, may be able to enter into a state of anhydrobiosis, in which their metabolism is temporarily and reversibly suspended or delayed. This evolutionarily developed adaptation to extreme conditions of the environment is widely used for practical goals - for conservation of microorganisms in collections, for maintenance and long storage of different important strain-producers and for other various biotechnological purposes. This current review presents the most important data obtained mainly in the studies of the structural and functional changes in yeast cells during dehydration. It describes the changes of the main organelles of eukaryotic cells and their role in cell survival in a dry state. The review provides information regarding the role of water in the structure and functions of biological macromolecules and membranes. Some important intracellular protective reactions of eukaryotic organisms, which were revealed in these studies and may have more general importance, are also discussed. The results of the studies of yeast anhydrobiosis summarized in the review show the possibilities of improving the conservation and long-term storage of various microorganisms and of increasing the quality of industrially produced dry microbial preparations.
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2.
Sodium and water handling during hemodialysis: new pathophysiologic insights and management approaches for improving outcomes in end-stage kidney disease.
Canaud, B, Kooman, J, Selby, NM, Taal, M, Francis, S, Kopperschmidt, P, Maierhofer, A, Kotanko, P, Titze, J
Kidney international. 2019;(2):296-309
Abstract
Space medicine and new technology such as magnetic resonance imaging of tissue sodium stores (23NaMRI) have changed our understanding of human sodium homeostasis and pathophysiology. It has become evident that body sodium comprises 3 main components. Two compartments have been traditionally recognized, namely one that is circulating and systemically active via its osmotic action, and one slowly exchangeable pool located in the bones. The third, recently described pool represents sodium stored in skin and muscle interstitium, and it is implicated in cell and biologic activities via local hypertonicity and sodium clearance mechanisms. This in-depth review provides a comprehensive view on the pathophysiology and existing knowledge gaps of systemic hemodynamic and tissue sodium accumulation in dialysis patients. Furthermore, we discuss how the combination of novel technologies to quantitate tissue salt accumulation (e.g., 23NaMRI) with devices to facilitate the precise attainment of a prescribed hemodialytic sodium mass balance (e.g., sodium and water balancing modules) will improve our therapeutic approach to sodium management in dialysis patients. While prospective studies are required, we think that these new diagnostic and sodium balancing tools will enhance our ability to pursue more personalized therapeutic interventions on sodium and water management, with the eventual goal of improving dialysis patient outcomes.
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3.
Aquaporins as a link between water relations and photosynthetic pathway in abiotic stress tolerance in plants.
Pawłowicz, I, Masajada, K
Gene. 2019;:166-172
Abstract
Plant aquaporins constitute a large family of proteins involved in facilitating the transport of water and small neutral molecules across biological membranes. In higher plants they are divided into several sub-families, depending on membrane-type localization and permeability to specific solutes. They are abundantly expressed in the majority of plant organs and tissues, and play a function in primary biological processes. Many studies revealed the significant role of aquaporins in acquiring abiotic stresses' tolerance. This review focuses on aquaporins belonging to PIPs sub-family that are permeable to water and/or carbon dioxide. Isoforms transporting water are involved in hydraulic conductance regulation in the leaves and roots, whereas those transporting carbon dioxide control stomatal and mesophyll conductance in the leaves. Changes in PIP aquaporins abundance/activity in stress conditions allow to maintain the water balance and photosynthesis adjustment. Broad analyses showed that tight control between water and carbon dioxide supplementation mediated by aquaporins influences plant productivity, especially in stress conditions. Involvement of aquaporins in adaptation strategies to dehydrative stresses in different plant species are discussed in this review.
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4.
Embracing 3D Complexity in Leaf Carbon-Water Exchange.
Earles, JM, Buckley, TN, Brodersen, CR, Busch, FA, Cano, FJ, Choat, B, Evans, JR, Farquhar, GD, Harwood, R, Huynh, M, et al
Trends in plant science. 2019;(1):15-24
Abstract
Leaves are a nexus for the exchange of water, carbon, and energy between terrestrial plants and the atmosphere. Research in recent decades has highlighted the critical importance of the underlying biophysical and anatomical determinants of CO2 and H2O transport, but a quantitative understanding of how detailed 3D leaf anatomy mediates within-leaf transport has been hindered by the lack of a consensus framework for analyzing or simulating transport and its spatial and temporal dynamics realistically, and by the difficulty of measuring within-leaf transport at the appropriate scales. We discuss how recent technological advancements now make a spatially explicit 3D leaf analysis possible, through new imaging and modeling tools that will allow us to address long-standing questions related to plant carbon-water exchange.
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5.
How do stomata respond to water status?
Buckley, TN
The New phytologist. 2019;(1):21-36
Abstract
Stomatal responses to humidity, soil moisture and other factors that influence plant water status are critical drivers of photosynthesis, productivity, water yield, ecohydrology and climate forcing, yet we still lack a thorough mechanistic understanding of these responses. Here I review historical and recent advances in stomatal water relations. Clear evidence now implicates a metabolically mediated response to leaf water status ('hydroactive feedback') in stomatal responses to evaporative demand and soil drought, possibly involving abscisic acid production in leaves. Other hypothetical mechanisms involving vapor and heat transport within leaves may contribute to humidity, light and temperature responses, but require further theoretical clarification and experimental validation. Variation and dynamics in hydraulic conductance, particularly within leaves, may contribute to water status responses. Continuing research to fully resolve mechanisms of stomatal responses to water status should focus on several areas: validating and quantifying the mechanism of leaf-based hydroactive feedback, identifying where in leaves water status is actively sensed, clarifying the role of leaf vapor and energy transport in humidity and temperature responses, and verifying foundational but minimally replicated results of stomatal hydromechanics across species. Clarity on these matters promises to deliver modelers with a tractable and reliable mechanistic model of stomatal responses to water status.
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6.
Exploring Dynamics and Structure of Biomolecules, Cryoprotectants, and Water Using Molecular Dynamics Simulations: Implications for Biostabilization and Biopreservation.
Weng, L, Stott, SL, Toner, M
Annual review of biomedical engineering. 2019;:1-31
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Abstract
Successful stabilization and preservation of biological materials often utilize low temperatures and dehydration to arrest molecular motion. Cryoprotectants are routinely employed to help the biological entities survive the physicochemical and mechanical stresses induced by cold or dryness. Molecular interactions between biomolecules, cryoprotectants, and water fundamentally determine the outcomes of preservation. The optimization of assays using the empirical approach is often limited in structural and temporal resolution, whereas classical molecular dynamics simulations can provide a cost-effective glimpse into the atomic-level structure and interaction of individual molecules that dictate macroscopic behavior. Computational research on biomolecules, cryoprotectants, and water has provided invaluable insights into the development of new cryoprotectants and the optimization of preservation methods. We describe the rapidly evolving state of the art of molecular simulations of these complex systems, summarize the molecular-scale protective and stabilizing mechanisms, and discuss the challenges that motivate continued innovation in this field.
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7.
The role of water in amyloid aggregation kinetics.
Stephens, AD, Kaminski Schierle, GS
Current opinion in structural biology. 2019;:115-123
Abstract
The role of water in protein function and aggregation is highly important and may hold some answers to understanding initiation of misfolding diseases such as Parkinson's, Alzheimer's and Huntington's where soluble intrinsically disordered proteins (IDPs) aggregate into fibrillar structures. IDPs are highly dynamic and have larger solvent exposed areas compared to globular proteins, meaning they make and break hydrogen bonds with the surrounding water more frequently. The mobility of water can be altered by presence of ions, sugars, osmolytes, proteins and membranes which differ in different cell types, cell compartments and also as cells age. A reduction in water mobility and thus protein mobility enhances the probability that IDPs can associate to form intermolecular bonds and propagate into aggregates. This poses an interesting question as to whether localised water mobility inside cells can influence the propensity of an IDP to aggregate and furthermore whether it can influence fibril polymorphism and disease outcome.
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8.
[Water Soluble Chlorophyll-Binding Proteins of Plants: Structure, Properties and Functions].
Maleeva, YV, Neverov, KV, Obukhov, YN, Kritsky, MS
Molekuliarnaia biologiia. 2019;(6):998-1011
Abstract
Water soluble chlorophyll-binding proteins (WSCPs) of higher plants differ from most proteins containing chlorophyll orbacteriochlorophyll in that they are soluble in watr and are neither embedded in the lipid membrane nor directly involved in the process of photosynthesis. Chlorophyll molecules in WSCPs ensembles are packed in dimers within the hydrophobic zone of the protein matrix, similar to the structure of a chlorophyll "special pair" in the reaction centers of phototrophs. This fact together with the detected photosensitizing activity of WSCPs makes it possible to consider these proteins as a promising object for modelling the evolutionary prototypes of the photosynthetic apparatus, as well as for developing the artificial solar energy converters. There are two classes of proteins in the WSCP family, class I and class II the representatives of these classes have a weak degree of homology in the primary structure, but a high degree of similarity in the tertiary and quaternary structure. One of the features of class I WSCPs is photoconversion, that is, to change the structure and spectral properties of the chromophore under the action of light. The functions of WSCPs in the plant are thought to be associated with stress protection.
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9.
The behavior of ions in water is controlled by their water affinity.
Collins, KD
Quarterly reviews of biophysics. 2019;:e11
Abstract
The strong, long-range electrostatic forces described by Coulomb's law disappear for ions in water, and the behavior of these ions is instead controlled by their water affinity - a weak, short-range force which arises from their charge density. This was established experimentally in the mid-1980s by size-exclusion chromatography on carefully calibrated Sephadex® G-10 (which measures the effective volume and thus the water affinity of an ion) and by neutron diffraction with isotopic substitution (which measures the density and orientation of water molecules near the diffracting ion and thus its water affinity). These conclusions have been confirmed more recently by molecular dynamics simulations, which explicitly model each individual water molecule. This surprising change in force regime occurs because the oppositely charged ions in aqueous salt solutions exist functionally as ion pairs (separated by 0, 1 or 2 water molecules) as has now been shown by dielectric relaxation spectroscopy; this cancels out the strong long-range electrostatic forces and allows the weak, short-range water affinity effects to come to the fore. This microscopic structure of aqueous salt solutions is not captured by models utilizing a macroscopic dielectric constant. Additionally, the Law of Matching Water Affinity, first described in 1997 and 2004, establishes that contact ion pair formation is controlled by water affinity and is a major determinant of the solubility of charged species since only a net neutral species can change phases.
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10.
Cold water immersion settings for reducing muscle tissue temperature: a linear dose-response relationship.
Vromans, BA, Thorpe, RT, Viroux, PJ, Tiemessen, IJ
The Journal of sports medicine and physical fitness. 2019;(11):1861-1869
Abstract
BACKGROUND Although cold water immersion (CWI) is widely accepted and integrated as a recovery modality in sports practice, questions regarding its proposed working mechanisms remain. This study systematically reviews the existing literature on one the proposed mechanisms of CWI, its effect on muscle tissue temperature, and subsequently tries to identify a dose-response relationship in order to describe an optimal dose. EVIDENCE ACQUISITION A systematic literature search (PubMed and Sport Discus) was conducted in October 2017. Dose-response relationships were analyzed using linear regression while controlling for possible confounders (muscle measurement depth and immersion position). EVIDENCE SYNTHESIS A total of 10 studies, with a total of 104 participants, were included utilizing 26 different CWI protocols. Muscle tissue temperatures were reduced significantly by 24 CWI protocols. A significant, relationship with a medium effect size (r=0.51) was identified between muscle tissue temperature and CWI. The most optimal dose-response relationship, with a large effect size, (r=0.87) was described for CWI protocols using full-body immersion at a measurement depth of 30 mm (y = 4.051 x + 0.535). CONCLUSIONS CWI can decrease muscle tissue temperature significantly if a minimum CWI dose of 1.1 is applied, corresponding with an immersion of 11 minutes with a water temperature of 10 °C.