1.
Association of Dietary Micronutrient Intake with Pulmonary Tuberculosis Treatment Failure Rate: ACohort Study.
Xiong, K, Wang, J, Zhang, J, Hao, H, Wang, Q, Cai, J, Ma, A
Nutrients. 2020;(9)
Abstract
Malnutrition is associated with an increased risk of pulmonary tuberculosis (PTB) treatment failure. Currently, there is no effective adjunctive nutritional therapy. The current objective is to investigate the association of dietary micronutrient intake with PTB treatment outcome.A cohort study including 1834 PTB patients was conducted in Linyi, China. The dietary micronutrient intake was assessed through a three-day 24 h dietary recall questionnaire. The treatment outcome was assessed by combinations of sputum smear and computerized tomography results. A multivariate binary regression model was used to assess the associations. The final model was adjusted for potential confounding factors. A low intake of vitamin C (adjusted OR (95% CI): 1.80 (1.07, 3.04), Ptrend = 0.02) and Zn (adjusted OR (95% CI): 2.52 (1.25, 5.08), Ptrend = 0.02) was associated with a high treatment failure rate. In addition, a low intake of vitamin C and Mn was associated with a severe tuberculosis symptom, as indicated by a high TB score. A supplementation of vitamin C and Zn may be beneficial in PTB treatment. Previous meta-analysis of randomized controlled trials (RCTs) reported a null effect of Zn supplementation on PTB treatment. The effect of vitamin C supplementation should be investigated by RCTs.
2.
Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001.
Zou, W, Liu, L, Zhang, J, Yang, H, Zhou, M, Hua, Q, Chen, J
Journal of biotechnology. 2012;(1):42-8
Abstract
Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining. This model was capable of predicting quantitatively the growth of K. vulgare under L-sorbose fermentation conditions and the results agreed well with experimental data. Furthermore, phenotypic features, such as the defect in sulfate metabolism hampering the syntheses of L-cysteine, L-methionine, coenzyme A (CoA), and glutathione, were investigated and provided an explanation for the poor growth of K. vulgare in monoculture. The model presented here provides a validated platform that can be used to understand and manipulate the phenotype of K. vulgare to further improve 2-KLG production efficiency.
3.
[Protection of beta-carotene and vitamin C on the transformation of human lung fibroblasts cell induced by nickel sesquioxide].
Zhang, J, Liu, Y, Zhang, J, Zhu, S
Wei sheng yan jiu = Journal of hygiene research. 2000;(4):237-9
Abstract
In order to study the protection of beta-carotene and vitamin C against the transformation of human lung fibroblasts (HLF) cell induced by nickel sesquioxide, HLF cells were treated repeatedly by different concentrations of Ni2O3 in vitro. beta-carotene (5.4 mg/L) and vitamin C(1.8 mg/L) were added into the media containing Ni2O3(1.0 mg/L) respectively. The identification of malignancy of the transformation of HLF cell was carried out by the tests of ConA and the growth on semisolid agar culture. The results showed that Ni2O3 could induce the malignant transformation of HLF cell. The transformed cell proliferated rapidly. The transformed colonies exhibited in extensively random orientation and the cells were crossingover. The frequency of transformation showed a dose-response relation at the experimental concentrations. The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar. The frequencies of transformation of HLF cell exposed to beta-carotene and vitamin C were decreased significantly. The cells could not be agglutinated by ConA and not grow in semisolid agar. It was concluded that Ni2O3 could induce strongly the malignant transformation of HLF cell and might be carcinogenic to human. The protection of beta-carotene and vitamin C on the transformation of HLF cell induced by Ni2O3 was observed. The increase of foods riched in beta-carotene and vitamin C was suggested for workers exposed to nickel.