Hydrogen-rich water reduces inflammatory responses and prevents apoptosis of peripheral blood cells in healthy adults: a randomized, double-blind, controlled trial.
Sim, M, Kim, CS, Shon, WJ, Lee, YK, Choi, EY, Shin, DM
Scientific reports. 2020;10(1):12130
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Oxidative stress indicates a state where excessive reactive oxygen species (ROS) overwhelm the biological antioxidant capacity, leading to disruption of ROS homeostasis and cellular damage. The aim of this study was to investigate the effects of hydrogen-rich water (HW) consumption in healthy adults through the extensive analyses of antioxidant capacity, peripheral blood mononuclear cell subsets and their transcriptome profile and to compare the effects of HW consumption with those of plain water (PW) consumption. This study is a 4-week, parallel-designed, randomized, double-blind, and placebo-controlled trial. The enrolled participants were randomly assigned to either the PW group (n=19) or the HW group (n=22). Results show that four-week consumption of HW induced a substantial increase in the antioxidant capacity and a decrease in oxidative stress of DNAs, although no significant results were found in the comparison of an intervention (HW) and the placebo (PW) group. Furthermore, the frequencies of apoptotic cells were significantly reduced by HW. Authors conclude that consumption of HW may promote biological antioxidant capacity for adults >30 years more than younger individuals.
The evidence for the beneficial effects of drinking hydrogen-water (HW) is rare. We aimed to investigate the effects of HW consumption on oxidative stress and immune functions in healthy adults using systemic approaches of biochemical, cellular, and molecular nutrition. In a randomized, double-blind, placebo-controlled study, healthy adults (20-59 y) consumed either 1.5 L/d of HW (n = 20) or plain water (PW, n = 18) for 4 weeks. The changes from baseline to the 4th week in serum biological antioxidant potential (BAP), derivatives of reactive oxygen, and 8-Oxo-2'-deoxyguanosine did not differ between groups; however, in those aged ≥ 30 y, BAP increased greater in the HW group than the PW group. Apoptosis of peripheral blood mononuclear cells (PBMCs) was significantly less in the HW group. Flow cytometry analysis of CD4+, CD8+, CD20+, CD14+ and CD11b+ cells showed that the frequency of CD14+ cells decreased in the HW group. RNA-sequencing analysis of PBMCs demonstrated that the transcriptomes of the HW group were clearly distinguished from those of the PW group. Most notably, transcriptional networks of inflammatory responses and NF-κB signaling were significantly down-regulated in the HW group. These finding suggest HW increases antioxidant capacity thereby reducing inflammatory responses in healthy adults.
Mechanical properties and microstructure analysis of mineral trioxide aggregate mixed with hydrophilic synthetic polymer.
Noh, YS, Chung, SH, Bae, KS, Baek, SH, Kum, KY, Lee, WC, Shon, WJ, Rhee, SH
Journal of biomedical materials research. Part B, Applied biomaterials. 2015;(4):777-82
INTRODUCTION In dentistry, mineral trioxide aggregate (MTA) has been widely used for root perforation, retrograde filling, pulp capping and regenerative endodontics. Despite its superior sealing ability and biocompatibility, MTA has critical drawbacks regarding handling property such as sandy property, lacking cohesive properties and wash-out tendency. So, it is necessary to improve the fluidity of MTA in order to improve its handling properties. In this study, we applied modified liquid to improve handling properties of MTA. METHODS Polyvinyl alcohol (PVA; 3 and 5 wt %) aqueous solutions were prepared and the samples were divided into three groups: DW group (MTA mixed with distilled water), P3 group (MTA mixed with 3% PVA), and P5 group (MTA mixed with 5% PVA). Handling property, initial setting time, and compressive strength were evaluated. The microstructures were observed by Field emission scanning electron microscope (FE-SEM) and X-ray diffractometer (XRD) phase analyses were performed. RESULTS PVA modified group showed similar behavior of IRM compared to DW group. The initial setting time of P3 or P5 group was significantly longer than that of DW group (p < 0.05). The compressive strength of DW group was higher than that of P3 or P5 groups (p < 0.05). Experimental groups (P3 and P5) showed no microstructural differences compared with DW group when the fractured surfaces were observed by FE-SEM with XRD patterns after 3 and 14 days. CONCLUSIONS Polyvinyl alcohol, a modified liquid for MTA, improved the handling properties of the material without violating its microstructure.
Effects of calcium phosphate endodontic sealers on the behavior of human periodontal ligament fibroblasts and MG63 osteoblast-like cells.
Shon, WJ, Bae, KS, Baek, SH, Kum, KY, Han, AR, Lee, WC
Journal of biomedical materials research. Part B, Applied biomaterials. 2012;(8):2141-7
In regard to biological properties of endodontic sealers, there are many characteristics that should be considered. The aim of this study was to examine the biological effects of new calcium phosphate-based root canal sealers, CAPSEAL I and CAPSEAL II (CPS), on human periodontal fibroblast cells by examining the expression levels of inflammatory mediators and to compare the effects of CPS on the viability and osteogenic potential of human osteoblast MG63 cells compared to those of other commercially available calcium phosphate sealers [Apatite Root Sealer type I (ARS I) and Apatite Root Sealer III (ARS III); Sankin Kogyo, Tokyo, Japan] and a zinc oxide eugenol-based sealer (Pulp Canal Sealer EWT [PCS EWT]; Kerr, Detroit, MI). The levels of IL-6 in the new CPS group (CAPSEAL I, II) were higher than those in the control and all experimental groups at all time points after 2 h. TGF-β1 and FGF-1 levels decreased at 72 h compared to the levels in the control, in cells treated with every sealers except ARS I. The new CPS sealers showed low cytotoxicity. Reverse transcription polymerase chain reaction showed that CAPSEAL I, II, and Apatite Root Sealer type III induced expression of early stage markers of differentiation (alkaline phosphatase and osteopontin) at 7 days. Also, new CPS showed higher mineralized nodule formation at 28 days. These results suggest that CAPSEAL I and II facilitate the periapical dentoalveolar and alveolar healing by controlling cellular mediators from PDL cells and osteoblast differentiation of precursor cells.