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FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells.
Penter, L, Dietze, K, Bullinger, L, Westermann, J, Rahn, HP, Hansmann, L
European journal of immunology. 2018;(7):1248-1250
Abstract
FACS index sorting allows the isolation of single cells with retrospective identification of each single cell's high-dimensional immune phenotype. We experimentally determine the error rate of index sorting and combine the technology with T cell receptor sequencing to identify clonal T cell expansion in aplastic anemia bone marrow as an example.