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In situ effects of Lathyrus sativus- PGPR to remediate and restore quality and fertility of Pb and Cd polluted soils.

Laboratory of Legumes, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia; National Agronomic Institute of Tunisia, University of Carthage, Tunis, Tunisia. Laboratory of Legumes, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia. Laboratory of Extremophile Plants, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia. Laboratory of Plant Molecular Physiology, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia. Laboratory of Legumes, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia. Electronic address: moez.jbara@cbbc.rnrt.tn.

Ecotoxicology and environmental safety. 2020;:110260
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Abstract

Rehabilitation of heavy metals contaminated soils using association between legumes and beneficial rhizospheric microorganisms such as plant growth-promoting bacteria (PGPR) is a major challenge in agronomy. The present study focuses on assessing the impact of field inoculation with I1 (Rhizobium leguminosarum (M5) + Bacillus simplex + Luteibacter sp. + Variovorax sp.) and I5 (R. leguminosarum (M5) + Pseudomonas fluorescens (K23) + Luteibacter sp. + Variovorax sp.) on growth and phytoremediation potential of Lathyrus sativus plants as well as soil quality and fertility. The experimentation was carried out in mine tailings of northern Tunisia. Obtained Results indicated that the in situ inoculation with I1 and I5 significantly increased the shoots (47% and 22%) and roots dry weights (22% and 29%), as well as nodules number (48% and 31%), respectively, compared to uninoculated plants. The maximum Pb accumulation in the above-ground tissue was recorded in plants inoculated with I5 (1180.85 mg kg-1 DW). At the same time, we noticed a reduction in total Pb and Cd in the rhizosphere of inoculated plots mainly in those inoculated with I5 reaching 46% and 61%, respectively, compared to uninoculated plots. Likewise, I5 inoculum significantly enhanced soil total nitrogen (35%) and available phosphorus (100%), as well as β-glucosidase (16%), urease (32%) and alkaline phosphatase (12%) activities. Here we demonstrate the usefulness of L. sativus inoculated with I5 inoculum formed by mixing efficient and heavy metals resistant PGPR to boost an efficient reclamation of Cd and Pb contaminated soils and, ultimately, to improve their quality and fertility.