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Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA; Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. Electronic address: paschalisdoulias@uoi.gr. Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA; Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA. Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA. Electronic address: ischirop@pennmedicine.upenn.edu.

Nitric oxide : biology and chemistry. 2021;:1-6
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Abstract

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.