-
1.
Anammox-based processes: How far have we come and what work remains? A review by bibliometric analysis.
Nsenga Kumwimba, M, Lotti, T, Şenel, E, Li, X, Suanon, F
Chemosphere. 2020;:124627
Abstract
Nitrogen contamination remains a severe environmental problem and a major threat to sustainable development worldwide. A systematic analysis of the literature indicates that the partial nitritation-anammox (PN/AMX) process is still actively studied as a viable option for energy-efficient and feasible technology for the sustainable treatment of N- rich wastewaters, since its initial discovery in 1990. Notably, the mainstream PN/AMX process application remains the most challenging bottleneck in AMX technology and fascinates the world's attention in AMX studies. This paper discusses the recent trends and developments of PN/AMX research and analyzes the results of recent years of research on the PN/AMX from lab-to full-scale applications. The findings would deeply improve our understanding of the major challenges under mainstream conditions and next-stage research on the PN/AMX process. A great deal of efforts has been made in the process engineering, PN/AMX bacteria populations, predictive modeling, and the full-scale implementations during the past 22 years. A series of new and excellent experimental findings at lab, pilot and full-scale levels including good nitrogen removal performance even under low temperature (15-10 °C) around the world were achieved. To date, pilot- and full-scale PN/AMX have been successfully used to treat different types of industrial sewage, including black wastewater, sludge digester liquids, landfill leachate, monosodium glutamate wastewater, etc. Supplementing the qualitative analysis, this review also provides a quantitative bibliometrics study and evaluates global perspectives on PN/AMX research published during the past 22 years. Finally, general trends in the development of PN/AMX research are summarized with the aim of conveying potential future trajectories. The current review offers a valuable orientation and global overview for scientists, engineers, readers and decision makers presently focusing on PN/AMX processes.
-
2.
Genome sequencing as a new window into the microbial community of membrane bioreactors - A critical review.
Nguyen, LN, Commault, AS, Kahlke, T, Ralph, PJ, Semblante, GU, Johir, MAH, Nghiem, LD
The Science of the total environment. 2020;:135279
Abstract
Recent developed sequencing techniques have resulted in a new and unprecedented way to study biological wastewater treatment, in which most organisms are uncultivable. This review provides (i) an insight on state-of-the-art sequencing techniques and their limitations; (ii) a critical assessment of the microbial community in biological reactor and biofouling layer in a membrane bioreactor (MBR). The data from high-throughput sequencing has been used to infer microbial growth conditions and metabolisms of microorganisms present in MBRs at the time of sampling. These data shed new insight to two fundamental questions about a microbial community in the MBR process namely the microbial composition (who are they?) and the functions of each specific microbial assemblage (what are their function?). The results to date also highlight the complexity of the microbial community growing on MBRs. Environmental conditions are dynamic and diverse, and can influence the diversity and structural dynamics of any given microbial community for wastewater treatment. The benefits of understanding the structure of microbial communities on three major aspects of the MBR process (i.e. nutrient removal, biofouling control, and micropollutant removal) were symmetrically delineated. This review also indicates that the deployment of microbial community analysis for a practical engineering context, in terms of process design and system optimization, can be further realized.
-
3.
The roles and applications of chaotropes and kosmotropes in industrial fermentation processes.
Timson, DJ
World journal of microbiology & biotechnology. 2020;(6):89
Abstract
Chaotropicity has long been recognised as a property of some compounds. Chaotropes tend to disrupt non-covalent interactions in biological macromolecules (e.g. proteins and nucleic acids) and supramolecular assemblies (e.g. phospholipid membranes). This results in the destabilisation and unfolding of these macromolecules and assemblies. Unsurprisingly, these compounds are typically harmful to living cells since they act against multiple targets, comprising cellular integrity and function. Kosmotropes are the opposite of chaotropes and these compounds promote the ordering and rigidification of biological macromolecules and assemblies. Since many biological macromolecules have optimum levels of flexibility, kosmotropes can also inhibit their activity and can be harmful to cells. Some products of industrial fermentations, most notably alcohols, are chaotropic. This property can be a limiting factor on rates of production and yields. It has been hypothesised that the addition of kosmotropes may mitigate the chaotropicity of some fermentation products. Some microbes naturally adapt to chaotropic environments by producing kosmotropic compatible solutes. Exploitation of this in industrial fermentations has been hampered by scientific and economic issues. The cost of the kosmotropes and their removal during purification needs to be considered. We lack a complete understanding of the chemistry of chaotropicity and a robust, quantitative framework for estimating overall chaotropicities of mixtures. This makes it difficult to predict the amount of kosmotrope required to neutralise the chaotropicity. This review considers examples of industrial fermentations where chaotropicity is an issue and suggests possible mitigations.
-
4.
Effect of Cultivation Parameters on Fermentation and Hydrogen Production in the Phylum Thermotogae.
Lanzilli, M, Esercizio, N, Vastano, M, Xu, Z, Nuzzo, G, Gallo, C, Manzo, E, Fontana, A, d'Ippolito, G
International journal of molecular sciences. 2020;(1)
Abstract
The phylum Thermotogae is composed of a single class (Thermotogae), 4 orders (Thermotogales, Kosmotogales, Petrotogales, Mesoaciditogales), 5 families (Thermatogaceae, Fervidobacteriaceae, Kosmotogaceae, Petrotogaceae, Mesoaciditogaceae), and 13 genera. They have been isolated from extremely hot environments whose characteristics are reflected in the metabolic and phenotypic properties of the Thermotogae species. The metabolic versatility of Thermotogae members leads to a pool of high value-added products with application potentials in many industry fields. The low risk of contamination associated with their extreme culture conditions has made most species of the phylum attractive candidates in biotechnological processes. Almost all members of the phylum, especially those in the order Thermotogales, can produce bio-hydrogen from a variety of simple and complex sugars with yields close to the theoretical Thauer limit of 4 mol H2/mol consumed glucose. Acetate, lactate, and L-alanine are the major organic end products. Thermotagae fermentation processes are influenced by various factors, such as hydrogen partial pressure, agitation, gas sparging, culture/headspace ratio, inoculum, pH, temperature, nitrogen sources, sulfur sources, inorganic compounds, metal ions, etc. Optimization of these parameters will help to fully unleash the biotechnological potentials of Thermotogae and promote their applications in industry. This article gives an overview of how these operational parameters could impact Thermotogae fermentation in terms of sugar consumption, hydrogen yields, and organic acids production.
-
5.
Unfolding microbial community intelligence in aerobic and anaerobic biodegradation processes using metagenomics.
Tikariha, H, Purohit, HJ
Archives of microbiology. 2020;(6):1269-1274
Abstract
Environmental factors and available nutrients influence microbial communities, and with that, there exists a dynamic shift in community structure and hierarchy in wastewater treatment systems. Of the various factors, the availability and gradient of oxygen selectively enrich a typical microbial community and also form the community stratification which could be established through metagenomics studies. In recent years, metagenomics with various sets of bioinformatics tools has assisted in exploration and better insight into the organization and relation of the taxonomical and functional composition and associate physiological intelligence of the microbial communities. The microbial communities, under defined conditions acquire a typical hierarchy with flexible but active network of the metabolic route, which ensures the survival needs of every member residing in that community and their abundance. This knowledge of community functional organization defines the rule in designing and improving biodegradation processes in case of both aerobic and anaerobic systems.
-
6.
High-strength wastewater treatment using microbial biofilm reactor: a critical review.
Abdelfattah, A, Hossain, MI, Cheng, L
World journal of microbiology & biotechnology. 2020;(5):75
Abstract
Biofilm reactors retain microbial cells in the form of biofilm which is attached to free moving or fixed carrying materials, thus providing a high active biomass concentration and automatic liquid and solid separation. Nowadays, microbial biofilm reactors have been widely used in high-strength wastewater treatment where very high pollutant removal efficiency is required, which usually requires excessive space and aeration energy for conventional activated sludge-based treatment. This paper provides an overview of microbial biofilm reactors developed over the last half-century, including moving bed biofilm reactor (MBBR), trickling filter (TF) reactor, rotating biological contactor (RBC), membrane biofilm reactor (MBfR), passive aeration simultaneous nitrification and denitrification (PASND) biofilm reactor, for their applications in high-strength wastewater treatment of not only removing carbon, nitrogen, sulphur but also a variety of oxidized contaminants including perchlorate and bromate. Despite the advance of biofilm reactor that exhibits high resistance to excessive pollutants loading, its drawbacks both from engineering and microbiological point of view are reviewed. The future prospects of biofilm reactor are also discussed in this review paper.
-
7.
Role of ultrasound in assisted fermentation technologies for process enhancements.
Pawar, SV, Rathod, VK
Preparative biochemistry & biotechnology. 2020;(6):627-634
Abstract
Biological molecules are widely produced by fermentation technology using bacteria, fungi or yeast. Fermentation is a biochemical process wherein the rate of bioconversion is governed by the organisms involved. The growth of the organism is mainly limited by mass transfer rates of nutrients and gases that directly affect the product formation in fermentation. Attempts have been made to enhance the growth rate and yield using mutational, recombinant strain development approach at microbial level as well as fed batch and continuous processing approach at bioprocess level in the past. The growth rate of microbes can be accelerated by increased mass transfer rates and cell wall permeability with the use of controlled low frequency ultrasound irradiation. The present review provides insights into the application of acoustic cavitation in process intensification of fermentation approaches and the role of various factors involved are highlighted with typical examples.
-
8.
Design, application, and microbiome of sulfate-reducing bioreactors for treatment of mining-influenced water.
Habe, H, Sato, Y, Aoyagi, T, Inaba, T, Hori, T, Hamai, T, Hayashi, K, Kobayashi, M, Sakata, T, Sato, N
Applied microbiology and biotechnology. 2020;(16):6893-6903
Abstract
Sulfate-reducing bioreactors, also called biochemical reactors, represent a promising option for passive treatment of mining-influenced water (MIW) based on similar technology to aerobic/anaerobic-constructed wetlands and vertical-flow wetlands. MIW from each mine site has a variety of site-specific properties related to its treatment; therefore, design factors, including the organic substrates and inorganic materials packed into the bioreactor, must be tested and evaluated before installation of full-scale sulfate-reducing bioreactors. Several full-scale sulfate-reducing bioreactors operating at mine sites provide examples, but holistic understanding of the complex treatment processes occurring inside the bioreactors is lacking. With the recent introduction of high-throughput DNA sequencing technologies, microbial processes within bioreactors may be clarified based on the relationships between operational parameters and key microorganisms identified using high-resolution microbiome data. In this review, the test design procedures and precedents of full-scale bioreactor application for MIW treatment are briefly summarized, and recent knowledge on the sulfate-reducing microbial communities of field-based bioreactors from fine-scale monitoring is presented.Key points• Sulfate-reducing bioreactors are promising for treatment of mining-influenced water.• Various design factors should be tested for application of full-scale bioreactors.• Introduction of several full-scale passive bioreactor systems at mine sites.• Desulfosporosinus spp. can be one of the key bacteria within field-based bioreactors.
-
9.
Production, characteristics, and biotechnological applications of microbial xylanases.
Alokika, , Singh, B
Applied microbiology and biotechnology. 2019;(21-22):8763-8784
Abstract
Microbial xylanases have gathered great attention due to their biotechnological potential at industrial scale for many processes. A variety of lignocellulosic materials, such as sugarcane bagasse, rice straw, rice bran, wheat straw, wheat bran, corn cob, and ragi bran, are used for xylanase production which also solved the great issue of solid waste management. Both solid-state and submerged fermentation have been used for xylanase production controlled by various physical and nutritional parameters. Majority of xylanases have optimum pH in the range of 4.0-9.0 with optimum temperature at 30-60 °C. For biochemical, molecular studies and also for successful application in industries, purification and characterization of xylanase have been carried out using various appropriate techniques. Cloning and genetic engineering are used for commercial-level production of xylanase, to meet specific economic viability and industrial needs. Microbial xylanases are used in various biotechnological applications like biofuel production, pulp and paper industry, baking and brewing industry, food and feed industry, and deinking of waste paper. This review describes production, characteristics, and biotechnological applications of microbial xylanases.
-
10.
Biofilm reactors as a promising method for vitamin K (menaquinone-7) production.
Mahdinia, E, Demirci, A, Berenjian, A
Applied microbiology and biotechnology. 2019;(14):5583-5592
Abstract
Menaquinone-7 (MK-7) is the most potent subtype of vitamin K with extraordinarily high half-life in the circulatory system. Therefore, MK-7 plays a critical role in promoting human wellbeing today. Studies on MK-7 every year show more and more magnificent benefits of it in preventing cardiovascular diseases and osteoporosis to battling cancer cells, Alzheimer's and Parkinson's diseases. Thus, it needs to be supplemented to daily diet for accumulative and long-term benefits. Chemical synthesis of MK-7 produces a significant cis-isomer form of it, which has no biological activity. Fortunately, due to its key role in electron transfer in bacteria, trans-MK-7 is biosynthesized by especially Gram-positive strains mainly Bacillus genus. Concordantly, MK-7 could be produced via solid or liquid state fermentation strategies. In either regime, when static fermentation is applied in the absence of agitation and aeration, operational issues arise such as heat and mass transfer inefficiencies. Thus, scaling up the process becomes a challenge. On the other hand, studies have indicated that biofilm and pellicle formation that occur in static fermentations are key characteristics for extracellular MK-7 secretion. Therefore, this review covers the most recent discoveries of the therapeutic properties of MK-7 and optimization attempts at increasing its biosynthesis in different media compositions and effective growth parameters as well as the cutting-edge use of biofilm reactors where B. subtilis cells have the infrastructures to form mature biofilm formations on plastic composite supports. Biofilm reactors therefore can provide robust extracellular MK-7 secretion while simultaneously enduring high agitation and aeration rates, which then address the scale-up and operational issues associated with static fermentation strategies.