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1.
Response of human pulp capped with a bonding agent after bleeding control with hemostatic agents.
Accorinte, Mde L, Loguercio, AD, Reis, A, Muench, A, de Araújo, VC
Operative dentistry. 2005;(2):147-55
Abstract
PURPOSE This study evaluated the response of human pulps capped with a bonding agent after bleeding control with different hemostatic agents. MATERIAL AND METHODS Twenty-five Class II cavities were prepared in 25 caries-free human premolars scheduled for extraction due to orthodontic treatment. The pulp exposures were performed on the occlusal floor. The teeth were randomly divided into five groups. Groups 1-4 were capped with an adhesive system after hemostasis with different agents: Group 1--saline solution; 2--ferric sulfate; 3--2.5% NaOCl; 4--Ca(OH)2 solution. In Group 5, after hemostasis with saline solution, the pulp was capped with calcium hydroxide (control group). Then, ScotchBond Multi Purpose Plus was applied and the resin composite Z-100 placed incrementally according to the manufacturers' directions. After 60 days, the teeth were extracted and processed for light microscopic examination (HE) and the groups were categorized in a histological score system. The data were subjected to a non-parametric test (alpha=0.05). RESULTS Overall, the histological features showed that the pulp response from Groups 1 through 4 was inferior to the response from Group 5, where dentin bridging occurred. In all groups, where the adhesive system was used for capping, the pulp response varied from an acute inflammatory, with varying degrees, to necrosis. No dentin bridge was formed after adhesive capping.
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2.
Ultrastructural correlates of in vivo/in vitro bond degradation in self-etch adhesives.
Donmez, N, Belli, S, Pashley, DH, Tay, FR
Journal of dental research. 2005;(4):355-9
Abstract
The morphologic correlates of bond degradation in self-etching primers have not been fully elucidated. We hypothesized that there is no difference between the mechanism of degradation of self-etching primers in vivo and in vitro. Class I cavities prepared in vivo in 24 caries-free human molars were bonded with Clearfil SE Bond or Clearfil Protect Bond, and restored with resin composites. Eight teeth were extracted after 24 hrs, and the rest after 1 yr. The same protocol was repeated in vitro with extracted molars. Degradation of resin-dentin bonds was assessed by microtensile bond testing and TEM of interfaces after tracer immersion. Both in vivo and in vitro bond strengths decreased with time for SE Bond but not for Protect Bond, with more pronounced water treeing observed in the former adhesive under both aging conditions. There is no difference between the mechanism of degradation of self-etch adhesives in vivo or in vitro.
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3.
Pulp tissue reactions to a dentin bonding agent as a direct capping agent.
Sübay, RK, Demirci, M
Journal of endodontics. 2005;(3):201-4
Abstract
The aim of this study was to investigate the response of human pulp tissue to a dentin bonding agent, Scotchbond Multi-Purpose Plus (SMPP), in exposed class V cavities. Sixteen human premolar teeth were mechanically exposed. Ten pulps were capped with SMPP and six teeth were capped with Dycal. The cavities were filled with a composite. After 40 days, the teeth were extracted and processed for histologic evaluation. Of the 10 teeth capped with SMPP, eight showed moderate chronic inflammation, one was severely inflamed, and one pulp had no to slight inflammation. None of the teeth capped with SMPP showed dentin bridge formation. Of the six teeth capped with Dycal, three exhibited incomplete dentin bridges associated with no to slight inflammation, and three showed no to slight inflammation, without formation of dentin bridges. Direct capping with Dycal with subsequent sealing with SMPP may show favorable results in pulp tissue. SMPP may cause inflammatory changes when applied directly to exposed pulp tissue.
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4.
Identification of collagen encapsulation at the dentin/adhesive interface.
Spencer, P, Wang, Y, Katz, JL
The journal of adhesive dentistry. 2004;(2):91-5
Abstract
PURPOSE The purpose of this study was to investigate the dentin/adhesive interfacial characteristics of three current commercial adhesives with different relative hydrophilic/hydrophobic composition, using a nondestructive staining technique. MATERIALS AND METHODS Dentin surfaces of 18 unerupted human third molars were randomly selected for treatment with one of three commercial dentin bonding agents according to manufacturers' instructions for the "wet" bonding technique. The adhesives were ranked based on hydrophilic/hydrophobic component ratios (ie, ability to dissolve in water), highest to lowest, as follows: Uno (Pulpdent) > Prime&Bond NT (PBNT, Dentsply Caulk) > Single Bond (SB, 3M ESPE). Dentin/adhesive (d/a) interface sections were stained with Goldner's trichrome, a classical bone stain, and examined using light microscopy. RESULTS The extent and degree to which the adhesive encapsulates the demineralized dentin matrix is reflected in the color differences in the stained sections. The depth of demineralization appeared comparable among these bonding systems, but adhesive infiltration varied from highest to lowest as follows: Uno > PBNT > SB. CONCLUSIONS The differential staining technique provided a clear representation of the depth of dentin demineralization and extent/degree of adhesive encapsulation of the exposed collagen at the d/a interface. This technique provides a mechanism for readily identifying vulnerable sites at the d/a interface. The composition of the one-bottle adhesive systems has a substantial effect on the interfacial structure of the d/a bond.
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5.
Effect of sodium hypochlorite on dentin bonding in primary teeth.
Correr, GM, Puppin-Rontani, RM, Correr-Sobrinho, L, Sinhoret, MA, Consani, S
The journal of adhesive dentistry. 2004;(4):307-12
Abstract
PURPOSE The aim of this study was to evaluate the effect of sodium hypochlorite (NaOCl) on the shear bond strength (SBS) using three bonding systems in primary dentin. MATERIALS AND METHODS Forty-five sound extracted primary molars were selected. The crowns were longitudinally sectioned, embedded in polystyrene resin, and flattened until a dentin surface was reached. The samples were assigned to 6 groups (n = 15): G1, Single Bond (SB); G2, NaOCl + SB; G3, Prime & Bond 2.1 (PB); G4, NaOCl + PB; G5, Clearfil SE Bond (CSE); G6, NaOCl + CSE. All the adhesive systems were applied according to the manufacturers' instructions, except for the application of 10% NaOCl solution for 60 s in groups 2, 4 (after acid etching), and 6 (before applying adhesive system). The composite resin was placed in increments in a mold and light cured for 20 s. The samples were stored in distilled water at 37 degrees C for 24 h and submitted to SBS testing with a crosshead speed of 0.5 mm/min. The failure sites were observed with SEM. The data were treated with ANOVA and Tukey's tests (p < 0.05). RESULTS There was no statistically significant difference between the groups with or without treatment of the substrate with NaOCl, regardless the material used. The SBS averages in MPa (+/-SD) were: G1: 15.8(1.9)a; G2: 14.6(1.3)a; G3: 10.2(0.7)a; G4: 9.9(0.2)a; G5: 13.3(1.2)a; and G6: 10.7(1.0)a. There was a statistically significant difference between the materials (SB > or = CSE > or = PB). Mixed failure was the failure type most frequently observed for all groups. CONCLUSION Dentin surface treatment with NaOCl did not affect the resin-dentin bonding strength in primary teeth.
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6.
Comparison of using calcium hydroxide or a dentine primer for reducing dentinal pain following crown preparation: a randomized clinical trial with an observation time up to 30 months.
Wolfart, S, Wegner, SM, Kern, M
Journal of oral rehabilitation. 2004;(4):344-50
Abstract
The purpose of this study was to evaluate effect of a calcium hydroxide suspension [Ca(OH)2] or glutaraldehyde based dentine primer (GDP) to reduce tooth sensitivity after full crown preparation. Thirty-six patients were treated with Ca(OH)(2) on one tooth and with GDP on another. Patients completed a questionnaire regarding sensitivity during crown preparation, when the not-anaesthetized abutments were irritated [cotton pellet (20 degrees C)]. The teeth were tested before (T(0)) and after using the desensitizer (T(1)), again after 7 days (T(2)), 6 months (T(3)) and 30 months (T(4)). The results were registered on a visual analogue scale [0 (no pain)-100 (severe pain)]. The changes of tooth sensitivity between different testing times were analysed. The median and the (interquartile range) for the different time intervals for Ca(OH)(2) were DeltaT(0)-T(1): 5 (6-17), DeltaT(0)-T(2): 17 (14-32), and for GDP, DeltaT(0)-T(1): 9 (7-18) DeltaT(0)-T(2): 18 (16-33), the decreases in sensitivity were lower for DeltaT(0)-T(1) than for DeltaT(0)-T(2) for both desensitizers (P < 0.001). There was no statistical difference (P > 0.05) between the agents (DeltaT(0)-T(1), DeltaT(0)-T(2), DeltaT(3)-T(4)). Both substances might be useful in reducing tooth sensitivity after crown preparation, but no differences in the efficacy were found when comparing the materials.
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7.
Effect of medications for root canal treatment on bonding to root canal dentin.
Erdemir, A, Ari, H, Güngüneş, H, Belli, S
Journal of endodontics. 2004;(2):113-6
Abstract
Use of resin-based restorative materials recently has become widely accepted for treatment of endodontically treated teeth. However, some solutions routinely used during endodontic treatment procedures may have an effect on bond strengths of adhesive materials to root canal dentin. The purpose of this in vitro study was to evaluate the effect of various medications on microtensile bond strength to root canal dentin. Fourteen extracted human single-rooted teeth were used. The crowns and the pulp tissues were removed. The root canals were then instrumented and widened to the same size. The teeth were randomly divided into seven groups of two teeth each. The root canal dentin walls of the roots were treated with 5% sodium hypochloride (NaOCI), 3% hydrogen peroxide (H2O2), the combination of H2O2 and NaOCl, or 0.2% chlorhexidine gluconate for 60 s; or calcium hydroxide or formocresol for 24 h. The teeth in control group were irrigated with water. The root canals were obturated using C&B Metabond. After 24 h of storage in distilled water, serial 1-mm-thick cross-sections were cut, and approximately 12 samples were obtained from each group. Microtensile bond strengths to root canal dentin were then measured by using an Instron machine. The data were recorded and expressed as MPa. The results indicated that NaOCI, H2O2, or a combination of NaOCl and H2O2 treatment decreased bond strength to root canal dentin significantly (p < 0.05). The teeth treated with chlorhexidine solution showed the highest bond strength values (p < 0.05). In conclusion, chlorhexidine is an appropriate irrigant solution for root canal treatment before adhesive post core applications.
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8.
Evaluation of a novel dentin bonding system compared to commercial bonding system.
Soeno, K, Suzuki, S, Yokomichi, R, Taira, Y, Atsuta, M
Journal of dentistry. 2004;(4):315-20
Abstract
OBJECTIVE The aim of the current study was to compare the bond strength of an experimental bonding system with commercial bonding systems. MATERIAL AND METHODS An aqueous solution of 10% ascorbic acid and 5% ferric chloride was prepared as an experimental conditioner (Exp). The flattened human dentin surfaces were conditioned with phosphoric acid, NaOCl, and Exp. They were bonded to a stainless steel rod with 4-META/MMA-TBB resin. Super-Bond C&B (10-3), Panavia Fluoro Cement (Panavia), Panavia Fluoro Cement with AD gel (AD/Panavia), and All-Bond 2 system (All-Bond 2) were also used. After immersion in water for 24 h, half of the specimens of each group were tested for tensile bond strength, and the remaining specimen groups were subjected to 10,000 cycles of thermo-cycling prior to tensile testing. All of the data were analysed by ANOVA and a post hoc test. In addition, two thermo-cycled specimens (10-3/10,000, Exp/10,000) were prepared for photomicrography. RESULTS The bond strengths of Exp and AD/Panavia groups showed no decrease after 10,000 thermo-cycles, although those of 10-3, Panavia, and All-Bond groups decreased significantly after thermo-cycling. SEM micrographs exhibited that cracks and voids can be observed in the 10-3/10,000 specimen at the interface of the adhesive resin and the hybrid layer, although neither a hybrid layer nor cracks can be seen in the Exp/10,000 specimen. CONCLUSION Comparison of the tensile bond strengths for all adhesive resins revealed that the novel dentin bond system with experimental conditioner and Panavia Fluoro Cement with AD gel exhibited the most durable bonding after thermo-cycling.
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9.
Reversal of dentin bonding to bleached teeth.
Kaya, AD, Türkün, M
Operative dentistry. 2003;(6):825-9
Abstract
Many studies have shown a considerable reduction in enamel bond strength of resin composite restorations when the bonding procedure is carried out immediately after bleaching. These studies claim that a certain waiting period is needed prior to restoration to reach the original bond strength values prior to bleaching. This study determined the effect of anti-oxidant applications on the bond strength values of resin composites to bleached dentin. Ninety human teeth extracted for orthodontic purposes were used in this study. The labial surface of each tooth was ground and flattened until dentin appeared. The polished surfaces were subjected to nine different treatments: 1) bleaching with gel (35% Rembrandt Virtuoso); 2) bleaching with gel + 10% sodium ascorbate (SA); 3) bleaching with gel + 10% butylhydroxyanisole (BHA); 4) bleaching with sol (35% hydrogen peroxide); 5) bleaching with sol + 10% sodium ascorbate; 6) bleaching with sol + 10% BHA; 7) bleaching with gel + immersed in artificial saliva for seven days; 8) bleaching with sol + immersed in artificial saliva for seven days; 9) no treatment. After bonding application, the resin composite in standard dimensions was applied to all specimens. The teeth were stored in distilled water at 37 degrees C for 24 hours and a universal testing machine determined their resistance to shear bond strength. The data was evaluated using ANOVA and Duncan tests. Bond strength in the bleached dentin group significantly decreased compared to the control group. On the other hand, the antioxidant treatment had a reversal effect on the bond strength to dentin. After the bleaching treatment, the 10% sodium ascorbate application was effective in reversing bond strength. In the samples where antioxidant was applied after the bleaching process, bonding strength in dentin tissue was at the same level as those teeth kept in artificial saliva for seven days.
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10.
Short-term evaluation of the pulpo-dentin complex response to a resin-modified glass-ionomer cement and a bonding agent applied in deep cavities.
Costa, CA, Giro, EM, do Nascimento, AB, Teixeira, HM, Hebling, J
Dental materials : official publication of the Academy of Dental Materials. 2003;(8):739-46
Abstract
OBJECTIVES To evaluate the response of the pulpo-dentin complex following application of a resin-modified glass-ionomer cement or an adhesive system in deep cavities performed in human teeth. METHODS Deep class V cavities were prepared on the buccal surface of 26 premolars. In Group 1 the cavity walls (dentin) and enamel were conditioned with 32% phosphoric acid and the dentin adhesive system One Step (Bisco, Inc., Itasca, IL, USA) was applied. In Groups 2 and 3, before total etching and application of bonding agent, the cavity floor was lined with the resin-modified glass-ionomer cement-Vitrebond (3M ESPE Dental Products Division, St. Paul, MN, USA) or the calcium hydroxide cement-Dycal (control group, Dentsply, Mildford, DE, USA), respectively. The cavities were restored using light-cured Z-100 composite resin (3M ESPE). The teeth were extracted between 5 and 30 days and prepared for microscopic assessment. Serial sections were stained with H/E, Masson's trichrome and Brown and Brenn techniques. RESULTS In Group 1, the inflammatory response was more evident than in Groups 2 and 3. Diffusion of dental material components across dentinal tubules was observed only in Group 1, in which the intensity of the pulp response increased as the remaining dentin thickness decreased. Bacteria were evidenced in the lateral walls of two samples (Group 2) which exhibited no inflammatory response or tissue disorganization. CONCLUSIONS Based on the experimental conditions, it was concluded total acid etching followed by application of One Step bonding agent cannot be recommended as adequate procedures. In this clinical condition the cavity walls should be lined with a biocompatible dental material, such as Vitrebond or Dycal.