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1.
Harnessing Biotechnology for the Development of New Seed Lipid Traits in Brassica.
Zhou, XR, Li, J, Wan, X, Hua, W, Singh, S
Plant & cell physiology. 2019;(6):1197-1204
Abstract
The seed oil quality of Brassica oilseed species has been improved in the last few decades, using conventional breeding approaches. Modern biotechnology has enabled the significant development of new seed lipid traits in many oil crops. Alternation of seed lipid component with gene knockout by RNAi gene silencing, artificial microRNA or gene editing within the crop is relative straightforward. Introducing a new pathway from an exogenous source via biotechnology enables the creation of a new trait, where the biosynthetic pathway for such a new trait is not available in the host crop. This review updates the recent development of new seed lipid traits in six major Brassica species and highlights the capability of biotechnology to improve the composition of important fatty acids for both industrial and nutritional purposes.
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2.
The signalling role of ROS in the regulation of seed germination and dormancy.
Bailly, C
The Biochemical journal. 2019;(20):3019-3032
Abstract
Reactive oxygen species (ROS) are versatile compounds which can have toxic or signalling effects in a wide range living organisms, including seeds. They have been reported to play a pivotal role in the regulation of seed germination and dormancy but their mechanisms of action are still far from being fully understood. In this review, we sum-up the major findings that have been carried out this last decade in this field of research and which altogether shed a new light on the signalling roles of ROS in seed physiology. ROS participate in dormancy release during seed dry storage through the direct oxidation of a subset of biomolecules. During seed imbibition, the controlled generation of ROS is involved in the perception and transduction of environmental conditions that control germination. When these conditions are permissive for germination, ROS levels are maintained at a level which triggers cellular events associated with germination, such as hormone signalling. Here we propose that the spatiotemporal regulation of ROS production acts in concert with hormone signalling to regulate the cellular events involved in cell expansion associated with germination.
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3.
Analysis of dihydroindole-type alkaloids in Strychnos nux-vomica unprocessed and processed seeds by high-performance liquid chromatography coupled with diode array detection and mass spectrometry.
Jiang, X, Tian, JX, Wang, M, Tian, Y, Zhang, ZJ
Journal of separation science. 2019;(22):3395-3402
Abstract
The ripened seeds of Strychnos nux-vomica L. have been extensively used as herbal medicines in Asian countries. Dihydroindole-type alkaloids are not only the active constituents but also the toxicants in Strychnos. However, the simultaneous determination of these alkaloids in both crude and processed Semen Strychni is still lacking. The present study represents the first quantitation and relative quantitation assay of 12 dihydroindole-type alkaloids in Strychnos nux-vomica unprocessed and sand-processed seeds using high-performance liquid chromatography coupled with diode array detection and mass spectrometry. The relative concentration of ten alkaloids was calculated by semi-quantification using the internal standard and their amounts in unprocessed and detoxified Semen Strychni were compared. We report here for the first time the significant increase of the two alkaloids, 19-N-methyl-strychnine, and 2,3-dimethoxy-19-N-methyl-strychnine, during the processing of Semen Strychni. Our study provides new insight into the true complexity of seed processing procedure and valuable information for assessing the efficacy and safety for clinical applications of Semen Strychni-containing drugs.
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4.
A brief note on genes that trigger components of apomixis.
Brukhin, V, Baskar, R
Journal of biosciences. 2019;(2)
Abstract
Apomixis or asexual reproduction through seeds occurs in about 400 species of flowering plants producing genetically uniform progeny. During apomixis, meiosis is bypassed and embryos develop by parthenogenesis. However, the endosperm could form either autonomously without fertilization or sexually, depending on the plant species. Most probably, a heterochronic expression of sexually expressed genes is one of the reason that causes apomixis. A better understanding of the genetic components regulating apomixis is important for developmental and evolutionary studies and also for engineering apomixis traits into crop plants that may realize a possibility to propagate hybrid vigor in a range of subsequent generations.
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5.
Phytochemistry, Pharmacological Activities, Toxicity and Clinical Application of Momordica cochinchinensis.
Lan, HY, Zhao, B, Shen, YL, Li, XQ, Wang, SJ, Zhang, LJ, Zhang, H
Current pharmaceutical design. 2019;(6):715-728
Abstract
Momordica cochinchinensis (Lour.) Spreng (M. cochinchinensis) is a deciduous vine that grows in Southeast Asia. It is known as gac in Vietnam and as Red Melon in English. Gac is reputed to be extremely benificial for health and has been widely used as food and folk medicine in Southeast Asia. In China, the seed of M. cochinchinensis (Chinese name: Mu biezi) is used as traditional Chinese medicine (TCM) for the treatment of various diseases. More than 60 chemical constituents have been isolated from M. cochinchinensis. Modern pharmacological studies and clinical practice demonstrate that some chemical constituents of M. cochinchinensis possess wide pharmacological activities, such as anti-tumor, anti-oxidation, anti-inflammatory, etc. This paper reviews the phytochemistry, pharmacological activities, toxicity, and clinical application of M. cochinchinensis, aiming to bring new insights into further research and application of this ancient herb.
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6.
Translating auxin responses into ovules, seeds and yield: Insight from Arabidopsis and the cereals.
Shirley, NJ, Aubert, MK, Wilkinson, LG, Bird, DC, Lora, J, Yang, X, Tucker, MR
Journal of integrative plant biology. 2019;(3):310-336
Abstract
Grain production in cereal crops depends on the stable formation of male and female gametes in the flower. In most angiosperms, the female gamete is produced from a germline located deep within the ovary, protected by several layers of maternal tissue, including the ovary wall, ovule integuments and nucellus. In the field, germline formation and floret fertility are major determinants of yield potential, contributing to traits such as seed number, weight and size. As such, stimuli affecting the timing and duration of reproductive phases, as well as the viability, size and number of cells within reproductive organs can significantly impact yield. One key stimulant is the phytohormone auxin, which influences growth and morphogenesis of female tissues during gynoecium development, gametophyte formation, and endosperm cellularization. In this review we consider the role of the auxin signaling pathway during ovule and seed development, first in the context of Arabidopsis and then in the cereals. We summarize the gene families involved and highlight distinct expression patterns that suggest a range of roles in reproductive cell specification and fate. This is discussed in terms of seed production and how targeted modification of different tissues might facilitate improvements.
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7.
Nanotherapeutic silibinin: An insight of phytomedicine in healthcare reformation.
Takke, A, Shende, P
Nanomedicine : nanotechnology, biology, and medicine. 2019;:102057
Abstract
Most of the herbal origin drugs possess water insoluble active constituents which lower the bioavailability and increase systemic clearance after administration of repeated or higher dose of drug. Silymarin is extracted from the seeds and fruits of milk thistle plant Silybum marianum which consists of main biologically active component as silibinin. However, the clinical applications of silibinin show some limitations due to low aqueous solubility, poor penetration into the epithelial cells of intestine, high metabolism and rapid systemic elimination. But nanotechnology-based drug delivery system explores great potential for phytochemicals to enhance the aqueous solubility and bioavailability of BCS class II and IV drugs, improve stability and modify the pharmacological activity. This review focuses on the therapeutic properties of silibinin and discusses the benefits, challenges and applications of silibinin nanoformulations. Such nanotherapeutic system as a regular medicine will be an attractive approach to reduce the adverse events and toxicities of current therapies.
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8.
The Chemical Composition and Nutritional Value of Chia Seeds-Current State of Knowledge.
Kulczyński, B, Kobus-Cisowska, J, Taczanowski, M, Kmiecik, D, Gramza-Michałowska, A
Nutrients. 2019;(6)
Abstract
Chia (Salvia hispanica) is an annual herbaceous plant, the seeds of which were consumed already thousands of years ago. Current research results indicate a high nutritive value for chia seeds and confirm their extensive health-promoting properties. Research indicates that components of chia seeds are ascribed a beneficial effect on the improvement of the blood lipid profile, through their hypotensive, hypoglycaemic, antimicrobial and immunostimulatory effects. This article provides a review of the most important information concerning the potential application of chia seeds in food production. The chemical composition of chia seeds is presented and the effect of their consumption on human health is discussed. Technological properties of chia seeds are shown and current legal regulations concerning their potential use in the food industry are presented.
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9.
A review of the seed biology of Paeonia species (Paeoniaceae), with particular reference to dormancy and germination.
Zhang, K, Yao, L, Zhang, Y, Baskin, JM, Baskin, CC, Xiong, Z, Tao, J
Planta. 2019;(2):291-303
Abstract
Most Paeonia species have epicotyl dormancy. Germination of peony seeds requires warm stratification for embryo growth and radicle protrusion followed by cold stratification for epicotyl growth. The genus Paeonia (Paeoniaceae) includes many popular ornamentals, has colorful flowers and contains several Chinese medicinal species. The germination protocol for seeds of Paeonia species is complex and impedes the breeding of new cultivars and contributes to the rarity and high cost of the plants. Although numerous reports on seed dormancy/germination in peonies are scattered throughout the literature, most of them are in Chinese. The primary aims of this paper are to provide a general overview of the available information on seed dormancy/germination in peonies and to make some suggestions regarding propagation for the peony industry and breeders. Most Paeonia species have epicotyl dormancy. The embryo is differentiated into organs, but it is underdeveloped (small) and must grow inside the seed before the radicle can emerge. Germination of peony seeds requires warm stratification for embryo growth and radicle protrusion followed by cold stratification for epicotyl growth. In addition, the epicotyl is sensitive to cold stratification only after the root has grown to a certain length. GA3 treatment enhances embryo growth and subsequent germination percentages. Further investigations on the physiology, genetics and proteomics would contribute to a better understanding of seed dormancy in Paeonia.
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10.
Concepts and strategies of soybean seed proteomics using the shotgun proteomics approach.
Min, CW, Gupta, R, Agrawal, GK, Rakwal, R, Kim, ST
Expert review of proteomics. 2019;(9):795-804
Abstract
Introduction: The last decade has yielded significant developments in the field of proteomics, especially in mass spectrometry (MS) and data analysis tools. In particular, a shift from gel-based to MS-based proteomics has been observed, thereby providing a platform with which to construct proteome atlases for all life forms. Nevertheless, the analysis of plant proteomes, especially those of samples that contain high-abundance proteins (HAPs), such as soybean seeds, remains challenging. Areas covered: Here, we review recent progress in soybean seed proteomics and highlight advances in HAPs depletion methods and peptide pre-fractionation, identification, and quantification methods. We also suggest a pipeline for future proteomic analysis, in order to increase the dynamic coverage of the soybean seed proteome. Expert opinion: Because HAPs limit the dynamic resolution of the soybean seed proteome, the depletion of HAPs is a prerequisite of high-throughput proteome analysis, and owing to the use of two-dimensional gel electrophoresis-based proteomic approaches, few soybean seed proteins have been identified or characterized. Recent advances in proteomic technologies, which have significantly increased the proteome coverage of other plants, could be used to overcome the current complexity and limitation of soybean seed proteomics.