-
1.
Promises and potential of in situ nano-phytoremediation strategy to mycorrhizo-remediate heavy metal contaminated soils using non-food bioenergy crops (Vetiver zizinoides & Cannabis sativa).
Khan, AG
International journal of phytoremediation. 2020;(9):900-915
Abstract
Heavy metals (HMs) in soil, air, and water environments effect human health. These HMs cannot be degraded in soil and they can only be transformed from one state to another. Food and energy resources such as coal, oil, petrol, etc. are gradually diminishing due to ever increasing demand and consumption, world faces crisis. There is an urgent need to address these problems by reclaiming the waste/polluted land for food and energy production. Various physicochemical remediation strategies are being proposed, developed, and tested but they are all very costly and only applicable to small contaminated sites. During the past two decades or so, plant-based phytoremediation technology is rapidly evolving as a promising new tool to address the issue with the potential to remediate HM contaminated soils in a sustainable manner. Plants, labeled as phyto-tolerant or phyto-accumulators, surviving on such contaminated soils reduce the toxicity by preventing their translocation or destroying the contaminants by sequestration by synthesizing thiol-containing HM-binding proteins (nano-molecules) and peptides (phytochelators or PCs) which modulate internal levels of metal concentration between deficient and toxic levels. But such plants are very slow growing, producing small biomass, and the process taking a long time to effectively remediate such soils. To overcome limitations of using such plants, plants capable of high biomass production and tolerating multiple HMs, such as non-food bioenergy crops (Vetiver and Hamp), are required. This plant-based remediation strategy can further be enhanced with the use of both plants and rhizosphere microbes like arbuscular mycorrhizal fungi (AMF) and plant growth-promoting bacteria. The combination of three components, i.e. high biomass producing plant, soil, and its rhizosphere harboring plant growth-promoting rhizobial (PGPR) microbiota, particularly AMF, will further improve the process of nano-phytoremediation of HM contaminated soils. This mini review focuses on how phytoremediation, nanotechnology, AMF and PGPR technologies can be merged together to form an integrated nano-mycorrhizo-phytoremediation (NMPR) strategy which synergistically achieve the goal of remediation of soil contaminants and improve the phytoremediation performance of bioenergy plants grown on HM polluted soils. This review also identifies the urgent need to conduct field-scale application of this strategy and use it as potential tool for reestablishing plant cover and population diversity during restoration of derelict land post-industrial/mining activities.
-
2.
Fast-stimulating bioremediation of macro crude oil in soils using matching Fenton pre-oxidation.
Xu, J, Du, J, Li, L, Zhang, Q, Chen, Z
Chemosphere. 2020;:126622
Abstract
This study aims at exploring the mechanism of fast-stimulating bioremediation of macro crude oil using matching Fenton pre-oxidation. The 80-day biodegradation experiment for soil S1 and S2, containing macro crude oil: C19-C29 and C17-C29 respectively, was conducted after Fenton pre-oxidation with three concentrations of H2O2 (225 mM, 450 mM, and 900 mM). Experimental results indicated that the bioremediation efficiency of macro crude oil was up to 57.1% (8853 mg/kg, S1) and 64.4% (11,719 mg/kg, S2) for 80-day fast-stimulating bioremediation using matching Fenton pre-oxidation (450 mM H2O2), which was 1.8-2.6 times that (S1: 22.2-37.1%; S2: 36.1-39.6%) for slow-stimulating bioremediation using un-matching Fenton pre-oxidation. Furthermore, the high-throughput analysis revealed that genera Sedimentibacter, Caenispirillum, and Brevundimonas became the dominant bacteria after matching Fenton pre-oxidation. Meanwhile, the highest logarithmic growth rate of indigenous hydrocarbon degraders (IHD) was obtained (S1: 64% and S2: 60%) for fast-stimulating bioremediation. And the consumption of NH4+-N was up to 90% and 94% in S1 and S2 within 60 days for fast-stimulating bioremediation, approximately 1.4 and 2.2 times that (S1: 65% and 62%; S2: 47% and 41%) for slow-stimulating remediation. The results showed that the macro crude oil became the main carbon source for IHD for the fast-stimulating bioremediation, resulting in the rapid growth of IHD. Thus, this study provides a fast and efficient remediation technology for bioremediation of macro crude oil-contaminated soils.
-
3.
Potential use of king grass (Pennisetum purpureum Schumach. × Pennisetum glaucum (L.) R.Br.) for phytoextraction of cadmium from fields.
Zhou, Z, Guo, Y, Hu, L, He, L, Xu, B, Huang, Z, Wang, G, Chen, Y
Environmental science and pollution research international. 2020;(28):35249-35260
Abstract
Using king grass (Pennisetum purpureum Schumach. × Pennisetum glaucum (L.) R.Br.) for phytoextraction is a promising technology for producing large amounts of biomass fuel while remediating contaminated soil. To assess the practical phytoextraction capacity of king grass, we conducted a field experiment with three different soil types (loam, sandy loam, clay loam) and cadmium (Cd) concentrations (0, 0.25, 0.5, 1, 2, 4, 8, and 16 mg kg-1, aged stably for 6 years). King grass were harvested at two different periods (elongation and maturity) to identify the optimal harvest time for extraction efficiency. The results showed that all treatments had bioconcentration factor (BCF) > 1 and translocation factor (TF) < 1; Cd is mainly stored in the roots. However, due to a high shoot biomass, the highest quantity of Cd extracted from shoots was 2.75 mg plant-1, from the experimental group with 16 mg kg-1 Cd added in sandy loam. A significant positive relationship (P < 0.05) was observed between the amount of Cd extracted from king grass stems, leaves, and roots from soil with the diethylene triamine pentacetate acid (DTPA) extractable Cd concentration. The Cd concentration in shoots at the maturity stage is lower than at the elongation stage, mainly due to the effect of biological dilution. Meanwhile, there is significantly more biomass (P < 0.05) at the maturity stage than at the elongation stage. At the latter, the extraction efficiency of the three soils was loam > sandy loam > clay loam, while at maturity it was sandy loam > clay loam > loam. This change in extraction efficiency can be attributed mainly to differences in soil DTPA-extractable Cd concentration and growth rate caused by differences in soil physical and chemical properties. According to calculations from multiple harvests using three types of soil, remediating contaminated soil with 0-16 mg kg-1 Cd would take 13.9-224.5 and 19.5-250.6 years, extracting 7.21-265.23 and 4.96-330.52 g ha-1 Cd while producing 33.62-66.50 and 73.8-110.5 t ha-1 dry biomass at the elongation (90 days) and maturity (120 days) stages, respectively. In summary, king grass has major potential for remediating Cd-contaminated soil while producing large volumes of biofuel.
-
4.
Uptake and transformation of decabromodiphenyl ether in different rice cultivars: Evidence from a carbon-14 study.
Zhao, P, Ye, Q, Yu, K, Whalen, JK, Rajesh Kumar, R, Cheng, X, Delgado-Moreno, L, Wang, W
The Science of the total environment. 2020;:135398
Abstract
The differences of PBDE absorption, accumulation, and metabolism in different cultivars of the same crop are rarely explored. This study used 14C tracing to fully demonstrate the uptake and transformation of soil-borne BDE209 in three rice cultivars, including two indica (HHZ and YD1) and one japonica cultivars (NJ3). Results showed that about 6.9, 17.2, and 17.4% of the applied 14C-BDE209 were transformed to 14C-metabolites in soils planted with HHZ, YD1, and NJ3, respectively. The 14C-BDE209 and its 14C-metabolites in soil could be absorbed by the rice and gradually transported to its root, stem, leaf, and grain, with the total whole-plant uptake of 8.52, 4.55 and 3.43 nmol for HHZ, YD1, and NJ3, respectively. The cultivar of HHZ had the greatest whole-plant 14C absorption but the lowest ΣPBDEs residues in its grain, with the ΣPBDEs of 421.8, 454.2 and 967.0 ng g-1 for HHZ, YD1, and NJ3, respectively. BDE-209 accounted for 90%, 31% and 50% of the ΣPBDEs in the grain from HHZ, YD1, and NJ3, respectively. The estimated daily intake (EDI) amounts of ΣPBDEs were 928, 1056, and 2675 ng kg-1 bw d-1 via consuming rice grains from HHZ, YD1, and NJ3, respectively, which were below the safe threshold limits for human consumption. This study proved the different BDE-209 absorption, accumulation and transformation in different rice cultivars, which potentially suggests the need of considering cultivar differences in assessing the dietary risks of PBDEs.
-
5.
Microbes involved in arsenic mobilization and respiration: a review on isolation, identification, isolates and implications.
Mazumder, P, Sharma, SK, Taki, K, Kalamdhad, AS, Kumar, M
Environmental geochemistry and health. 2020;(10):3443-3469
Abstract
Microorganisms play an important role in arsenic (As) cycling in the environment. Microbes mobilize As directly or indirectly, and natural/geochemical processes such as sulphate and iron reduction, oxidative sulphide mineral dissolution, arsenite (AsO33-) oxidation and arsenate (AsO43-) respiration further aid in As cycle in the environment. Arsenate serves as an electron donor for the microbes during anaerobic conditions in the sediment. The present work reviews the recent development in As contamination, various As-metabolizing microbes and their phylogenetic diversity, to understand the role of microbial communities in As respiration and mobilization. It also summarizes the contemporary understanding of the intricate biochemistry and molecular biology of natural As metabolisms. Some successful examples of engineered microbes by harnessing these natural mechanisms for effective remediation are also discussed. The study indicates that there is an exigent need to have a clear understanding of environmental aspects of As mobilization and subsequent oxidation-reduction by a suitable microbial consortium.
-
6.
Identification and Validation of Reference Genes for RT-qPCR Analysis in Switchgrass under Heavy Metal Stresses.
Zhao, J, Zhou, M, Meng, Y
Genes. 2020;(5)
Abstract
Switchgrass (Panicum Virgatum L.) has been recognized as the new energy plant, which makes it ideal for the development of phytoremediation on heavy metal contamination in soils with great potential. This study aimed to screen the best internal reference genes for the real-time quantitative PCR (RT-qPCR) in leaves and roots of switchgrass for investigating its response to various heavy metals, such as cadmium (Cd), lead (Pb), mercury (Hg), chromium (Cr), and arsenic (As). The stability of fourteen candidate reference genes was evaluated by BestKeeper, GeNorm, NormFinder, and RefFinder software. Our results identified U2AF as the best reference gene in Cd, Hg, Cr, and As treated leaves as well as in Hg, Pb, As, and Cr stressed root tissues. In Pb treated leaf tissues, 18S rRNA was demonstrated to be the best reference gene. CYP5 was determined to be the optimal reference gene in Cd treated root tissues. The least stable reference gene was identified to be CYP2 in all tested samples except for root tissues stressed by Pb. To further validate the initial screening results, we used the different sets of combinatory internal reference genes to analyze the expression of two metal transport associated genes (PvZIP4 and PvPDB8) in young leaves and roots of switchgrass. Our results demonstrated that the relative expression of the target genes consistently changed during the treatment when CYP5/UBQ1, U2AF/ACT12, eEF1a/U2AF, or 18S rRNA/ACT12 were combined as the internal reference genes. However, the time-dependent change pattern of the target genes was significantly altered when CYP2 was used as the internal reference gene. Therefore, the selection of the internal reference genes appropriate for specific experimental conditions is critical to ensure the accuracy and reliability of RT-qPCR. Our findings established a solid foundation to further study the gene regulatory network of switchgrass in response to heavy metal stress.
-
7.
Antibiotic-contaminated wastewater irrigated vegetables pose resistance selection risks to the gut microbiome.
Gudda, FO, Waigi, MG, Odinga, ES, Yang, B, Carter, L, Gao, Y
Environmental pollution (Barking, Essex : 1987). 2020;:114752
Abstract
Wastewater reuse in food crop irrigation has led to agroecosystem pollution concerns and human health risks. However, there is limited attention on the relationship of sub-lethal antibiotic levels in vegetables and resistance selection. Most risk assessment studies show non-significant toxicity, but overlook the link between antibiotics in crops and propagation of gut microbiome resistance selection. The review highlights the risk of antibiotics in treated water used for irrigation, uptake, and accumulation in edible vegetable parts. Moreover, it elucidates the risks to the adaptive resistance selection of the gut microbiome from sub-lethal antibiotic levels, as a result of dietary contaminated vegetables. Experiments have reported that bacterial resistance selection is possible at concentrations that are several hundred-folds lower than lethal effect levels on susceptible cells. Consequently, mutants selected at low antibiotic levels, such as those from vegetables, are fitter and more resistant compared to those selected at high concentrations. Necessary standardization, such as the development of minimum acceptable antibiotic limits allowable in food crop irrigation water, with a focus on minimum selection concentration, and not only toxicity, has been proposed. Wastewater irrigation offers environmental benefits and can contribute to food security, but it has non-addressed risks. Research gaps, future perspectives, and frameworks of mitigating the potential risks are discussed.
-
8.
The mechanisms of biochar interactions with microorganisms in soil.
Gorovtsov, AV, Minkina, TM, Mandzhieva, SS, Perelomov, LV, Soja, G, Zamulina, IV, Rajput, VD, Sushkova, SN, Mohan, D, Yao, J
Environmental geochemistry and health. 2020;(8):2495-2518
Abstract
Biochar, a carbonaceous material, is increasingly used in the remediation of the anthropogenically polluted soils and the restoration of their ecological functions. However, the interaction mechanisms among biochar, inorganic and organic soil properties and soil biota are still not very clear. The effect of biochar on soil microorganisms is very diverse. Several mechanisms of these interactions were suggested. However, a well acceptable mechanism of biochar effect on soil microorganisms is still missing. Therefore, efforts were made to examine and proposed a mechanism of the interactions between biochar and microorganisms, as well as existing problems of biochar impacts on main groups of soil enzymes, the composition of the microbiota and the detoxification (heavy metals) and degradation (polycyclic aromatic hydrocarbons) of soil pollutants. The data on the process of biochar colonization by microorganisms and the effect of volatile pyrolysis products released by biochar on the soil microbiota were analysed in detail. The effects of biochar on the physico-chemical properties of soils, the content of mineral nutrients and the response of microbial communities to these changes are also discussed. The information provided here may contribute to the solution of the feasibility, effectiveness and safety of the biochar questions to enhance the soil fertility and to detoxify pollutants in soils.
-
9.
Biological approaches of fluoride remediation: potential for environmental clean-up.
Katiyar, P, Pandey, N, Sahu, KK
Environmental science and pollution research international. 2020;(12):13044-13055
Abstract
Fluoride (F), anion of fluorine which is naturally present in soil and water, behaves as toxic inorganic pollutant even at lower concentration and needs immediate attention. Its interaction with flora, fauna and other forms of life, such as microbes, adversely affect various physiochemical parameters by interfering with several metabolic pathways. Conventional methods of F remediation are time-consuming, laborious and cost intensive, which renders them uneconomical for sustainable agriculture. The solution lies in cracking down this environmental contaminant by adopting economic, eco-friendly, cost-effective and modern technologies. Biological processes, viz. bioremediation involving the use of bacteria, fungi, algae and higher plants that holds promising alternative to manage F pollution, recover contaminated soil and improve vegetation. The efficiency of indigenous natural agents may be enhanced, improved and selected over the hazardous chemicals in sustainable agriculture. This review article emphasizes on various biological approaches for the remediation of F-contaminated environment, and exploring their potential applications in environmental clean-up. It further focuses on thorough systemic study of modern biotechnological approaches such as gene editing and gene manipulation techniques for enhancing the plant-microbe interactions for F degradation, drawing attention towards latest progresses in the field of microbial assisted treatment of F-contaminated ecosystems. Future research and understanding of the molecular mechanisms of F bioremediation would add on to the possibilities of the application of more competent strains showing striking results under diverse ecological conditions.
-
10.
Alteration of enzyme activities and functional diversity of a soil contaminated with copper and arsenic.
Aponte, H, Herrera, W, Cameron, C, Black, H, Meier, S, Paolini, J, Tapia, Y, Cornejo, P
Ecotoxicology and environmental safety. 2020;:110264
Abstract
Copper (Cu) mining has to address a critical environmental issue related to the disposal of heavy metals and metalloids (HMs). Due to their deleterious effects on living organisms, Cu and arsenic (As) have gained global attention, and thus their monitoring in the environment is an important task. The aims of this study were: 1) to evaluate the alteration of soil enzyme activities (EAs) and soil microbial functional diversity with Cu/As contamination, and 2) to select the most reliable biochemical indicators of Cu/As contamination. A twelve-week soil experiment was performed with four increasing levels of Cu, As, and Cu/As from 150/15 to 1000/100 mg Cu/As kg-1. Soil enzyme activities and soil community-level physiological profile (CLPP) using MicroResp™ were measured during the experiment. Results showed reduced EAs over time with increasing Cu and Cu/As levels. The most Cu-sensitive EAs were dehydrogenase, acid phosphatase, and arylsulfatase, while arginine ammonification might be related to the resilience of soil microbial communities due to its increased activity in the last experimental times. There was no consistent response to As contamination with reduced individual EAs at specific sampling times, being urease the only EA negatively affected by As. MicroResp™ showed reduced carbon (C) substrate utilization with increasing Cu levels indicating a community shift in C acquisition. These results support the use of specific EAs to assess the environmental impact of specific HMs, being also the first assessment of EAs and the use of CLPP (MicroResp™) to study the environmental impact in Cu/As contaminated soils.