1.
Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture.
Kuhar, F, Castiglia, VC, Zamora, JC
Revista Argentina de microbiologia. 2016;(4):274-278
Abstract
Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities.
2.
Comparative genomics of MAP kinase and calcium-calcineurin signalling components in plant and human pathogenic fungi.
Rispail, N, Soanes, DM, Ant, C, Czajkowski, R, Grünler, A, Huguet, R, Perez-Nadales, E, Poli, A, Sartorel, E, Valiante, V, et al
Fungal genetics and biology : FG & B. 2009;(4):287-98
-
-
Free full text
-
Abstract
Mitogen-activated protein kinase (MAPK) cascades and the calcium-calcineurin pathway control fundamental aspects of fungal growth, development and reproduction. Core elements of these signalling pathways are required for virulence in a wide array of fungal pathogens of plants and mammals. In this review, we have used the available genome databases to explore the structural conservation of three MAPK cascades and the calcium-calcineurin pathway in ten different fungal species, including model organisms, plant pathogens and human pathogens. While most known pathway components from the model yeast Saccharomyces cerevisiae appear to be widely conserved among taxonomically and biologically diverse fungi, some of them were found to be restricted to the Saccharomycotina. The presence of multiple paralogues in certain species such as the zygomycete Rhizopus oryzae and the incorporation of new functional domains that are lacking in S. cerevisiae signalling proteins, most likely reflect functional diversification or adaptation as filamentous fungi have evolved to occupy distinct ecological niches.