1.
[Effects on blood fat and bone density of postmenopausal women fed by soy protein with isoflavone].
Chen, J, Liu, X
Zhonghua yi xue za zhi. 2014;(3):215-7
Abstract
OBJECTIVE To explore the effects of phytoestrogen (PE) on blood lipid and bone density in postmenopausal women. METHODS A total of 75 menopausal women aged 50-70 years with estrogen reduction symptoms received an intake of soy protein containing 70 mg isoflavone daily in a year. Their changes of blood fat, density lumbar bone and sex hormone level were compared with control group without an intake. RESULTS The changes of blood triglyceride (TG), total cholesterol (TC) and low-density lipoprotein (LDL) in two groups before and after a year showed no statistical significance.High-density lipoprotein (HDL) decreased in control group while it had no significant change in the study group. Bone densities in two groups showed a downward trend by an annual rate of 1%-4%, the changes in two groups showed no statistical significance.E2 increased slightly over basic value in the study group. But it had no statistical significance. The changes of follicle-stimulating hormone (FSH) in two groups were also similar. CONCLUSION The above soy protein preparation has no effect on hypothalamic-pituitary-ovarian axis and no stimulation on endometrium of uterus. But it may improve the profile of HDL.
2.
Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography.
Zhang, Y, Chen, J, Zhang, C, Wu, W, Liang, X
The Journal of steroid biochemistry and molecular biology. 2005;(4):375-81
Abstract
The estrogenic activity of the Chinese herb kudzu root was investigated by a recombinant yeast screening assay (YES). Isoflavones are the main components in the plant, of which puerarin is the most abundant one. The kudzu root extract was separated into four fractions according to the polarity. The crude extract and its sub-fractions, except the water fraction, showed clear estrogenic activity and the potencies were in the range of 10(-3) to 10(-1)g/l. The ligand potency was used to compare the estrogenic activity of these fractions. The crude extract and its sub-fractions were further analyzed by high performance liquid chromatography (HPLC) to correlate the activity and the active components. Bioassay and chemical analysis showed that theoretical estrogenic activity expressed as equivalent 17beta-estradiol concentration or the cumulative effects are comparable to that experimentally determined by YES. The results showed that the high content of isoflavones as well as the high estrogenic activity could make kudzu root extract an interesting candidate for hormone replacement therapy.