1.
Theoretical Studies on DNA-Cleavage Mechanism of Copper(II) Complexes: Probing Generation of Reactive Oxygen Species.
Miao, T, Deng, Q, Gao, H, Fu, X, Li, S
Journal of chemical information and modeling. 2018;(4):859-866
Abstract
Theoretical studies on DNA-cleavage properties of [Cu(bba)(diimine)] 1-4 have been carried out using density functional theory (DFT) and docking methods. The optimized structures of Cu(II) complexes were docked into DNA, glutathiones (GSH), and ascorbic acids (VC) so that the corresponding docking models were obtained. To explore DNA-cleavage properties of Cu(II) complexes, the docking models of complexes with GSH and VC were further optimized using DFT method, while the docking models of complexes with DNA were optimized using QM/MM method because DNA is a supramolecular system. The rate constants ket between complexes and DNA, GSH, and VC, oxidation-reduction potentials of complexes, and binding energies of complexes with GSH and VC were computed. The DNA-cleavage abilities of Cu(II) complexes in the presence VC, GSH, and H2O2 were explored and the experimental results could be reasonably explained. Finally, the DNA-cleavage mechanism of Cu(II) complexes was described in detail, which would contribute to future design of novel anticancer Cu(II) complexes.
2.
Hydrogen peroxide biosensor based on microperoxidase-11 immobilized on flexible MWCNTs-BC nanocomposite film.
Zhang, B, Zhou, J, Li, S, Zhang, X, Huang, D, He, Y, Wang, M, Yang, G, Shen, Y
Talanta. 2015;:243-8
Abstract
In the present work, we report on an experimental study of flexible nanocomposite film for electrochemical detection of hydrogen peroxide (H2O2) based on bacterial cellulose (BC) and multi-walled carbon nanotubes (MWCNTs) in combination with microperoxidase-11 (MP-11). MWCNTs are used to functionalize BC and provide a flexible conductive film. On the other hand, BC can improve MWCNTs׳ biocompatibility. The investigation shows that MP-11 immobilized on the flexible film of MWCNTs-BC can easily present a pair of well-defined and quasi-reversible redox peaks, revealing a direct electrochemistry of MP-11 on the nanocomposite film. The apparent heterogeneous electron-transfer rate constant ks is estimated to be 11.5s(-1). The resulting flexible electrode presents appreciated catalytic properties for electrochemical detection of H2O2, comparing to traditional electrodes (such as gold, glassy carbon electrode) modified with MP-11. The proposed biosensor exhibits a low detection limit of 0.1 µM (at a signal-to-noise ratio of 3) with a linear range of 0.1-257.6 µM, and acquires a satisfactory stability.
3.
[Effects of H2O2 addition on oxygen supply and metabolism of microorganisms].
Li, S, Jiao, P, Cao, Z
Wei sheng wu xue bao = Acta microbiologica Sinica. 2002;(1):129-32