1.
Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry.
Liu, Y, Li, Y, Zhang, T, Zhao, H, Fan, S, Cai, X, Liu, Y, Li, Z, Gao, S, Li, Y, et al
Molecular medicine reports. 2020;(5):3862-3872
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Abstract
Unstable angina (UA) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non‑invasive techniques to identify urine biomarkers in patients with UA, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolomics, urine samples from 28 patients with UA and 28 healthy controls (HCs) were analyzed using ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑Q‑TOF/MS). A total of 16 significant biomarkers that could distinguish between patients with UA and HCs, including D‑glucuronic acid, creatinine, succinic acid and N‑acetylneuraminic acid, were identified. The major metabolic pathways associated with UA were subsequently analyzed by non‑targeted metabolomics. The results demonstrated that amino acid and energy metabolism, fatty acid metabolism, purine metabolism and steroid hormone biosynthetic metabolism may serve important roles in UA. The results of the current study may provide a theoretical basis for the early diagnosis of UA and novel treatment strategies for clinicians. The trial was registered with the Chinese Clinical Trial Registration Center (registration no. ChiCTR‑ROC‑17013957) at Tianjin University of Traditional Chinese Medicine.
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Plasma ghrelin concentrations are negatively correlated with urine albumin-to-creatinine ratio in newly diagnosed type 2 diabetes.
Ma, X, Zhao, Y, Wang, Q, Wu, L, Wang, Z, Ma, X, Ren, G, Zhang, Y, Li, Z, Lu, J, et al
The American journal of the medical sciences. 2014;(5):382-6
Abstract
BACKGROUND Aging is associated with a decrease in appetite, energy intake and glucose tolerance. Experimental studies have suggested that ghrelin and obestatin play a role in glucose homeostasis and in the regulation of energy metabolism. However, few studies have been performed on the role of ghrelin and obestatin in middle-aged and old adults. METHODS In the present study, we investigated the plasma concentrations of ghrelin and obestatin in middle-aged (41-64 years) and old (65-76 years) subjects with newly diagnosed type 2 diabetes mellitus (NDD) and normal glucose tolerance (NGT). We also characterized the relationship among plasma ghrelin and obestatin levels and glucose/lipid metabolism. The fasting plasma ghrelin and obestatin concentrations were analyzed using enzyme immunoassay method. RESULTS Plasma obestatin concentrations in diabetic subjects were significantly lower than those in NGT subjects. Plasma ghrelin were negatively associated with fasting glucose, hemoglobin A1c, urine albumin-to-creatinine ratio (UACR) and positively correlated with high-density lipoprotein cholesterol. In addition, plasma obestatin level was correlated negatively with systolic blood pressure, triglycerides and total cholesterol. Furthermore, multiple regression analysis indicated that UACR was a significantly independent predictor of fasting plasma ghrelin levels. CONCLUSIONS Collectively, ghrelin and obestatin levels may be markers reflecting glucose and lipid conditions in NDD. The lower ghrelin levels may be a potential indicator for renal dysfunction in patients with type 2 diabetes mellitus.
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Is cystatin C a better marker than creatinine for evaluating residual renal function in patients on continuous ambulatory peritoneal dialysis?
Yang, Q, Li, R, Zhong, Z, Mao, H, Fan, J, Lin, J, Yang, X, Wang, X, Li, Z, Yu, X
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 2011;(10):3358-65
Abstract
BACKGROUND Current clinical assessments of residual renal function (RRF) for continuous ambulatory peritoneal dialysis (CAPD) patients usually require 24 h of urine collection, which is sometimes difficult for patients and contributes to random errors. Objective. Our study aims to investigate whether serum cystatin C (CysC) can serve as a better marker of RRF than serum creatinine (Cr) in CAPD and to develop a formula to estimate RRF with CysC levels. METHODS One hundred and sixty CAPD patients from a single dialysis unit were randomly divided into modeling (n(1) = 120) and validation (n(2) = 40) groups. RRF was assessed as the average of the renal clearances of urea and creatinine. We then derived RRF formulas based on the CysC and Cr levels from the modeling group and validated them by comparison with a published CysC-based equation and Modification of Diet in Renal Disease formula. RESULTS CysC levels were inversely related to RRF, Kt/V(urea) and total weekly Ccr but were unrelated to age, gender, body mass index, diabetes or peritoneal clearance. The RRF formulas derived from CysC and Cr were (sinh(ln(6.736-0.566 CysC)))(2) and (sinh(ln(6.097-0.265 Cr)))(2), respectively. When applied to the validation group, the estimated RRF based on CysC (2.8 ± 1.2 mL/min/1.73 m(2)) was similar to that of on Cr (2.8 ± 1.3 mL/min/1.73 m(2)) and the measured RRF (2.9 ± 1.7 mL/min/1.73 m(2)). The CysC formula showed a small bias, with the best 30 and 50% accuracy and had a larger area under the curve and higher sensitivity and specificity when compared to the Cr formula and other formulas. CONCLUSION Serum CysC may be a good marker for the estimation of RRF in CAPD patients. The derived CysC formula may be used to reliably estimate RRF in CAPD patients without the need for collection of 24 h urine.