1.
A "turn-on" fluorescent probe for glutathione detection based on the polyethylenimine-carbon dots-Cu2+ system.
Zhang, B, Duan, Q, Li, Y, Zhang, Y, Che, M, Zhang, W, Sang, S
Journal of photochemistry and photobiology. B, Biology. 2019;:111532
Abstract
Glutathione (GSH) plays critical roles in many physiological processes usually present in live cells, and altered levels have been linked to some clinical pathological conditions. However, current techniques of GSH detection with fluorescence assay strategies remain poorly researched. In this work, branched polyethylenimine-functionalized carbon dots (PEI-CDs) are synthesized by simple hydrothermal treatment of glucose and PEI. The fluorescence of the PEI-CDs could be efficiently quenched by Cu2+ and then recovered by some biothiols. Basing on this, a "turn-on" fluorescent probe for detecting GSH has been developed using PEI-CDs-Cu2+ system. Compared with traditional probes for GSH detection, a significant advantage of the PEI-CDs-Cu2+ system is that it can be used for GSH detection at both low and high concentrations with different concentration combinations of PEI-CDs and Cu2+. More specifically, two good linear relationships are achieved in the ranges of 0-80 μM and 0-1400 μM for GSH, respectively. Correspondingly, the detection limits of GSH are 0.33 μM and 9.49 μM, respectively. The quantum yields (QYs) of PEI-CDs and PEI-CDs-Cu2++GSH was 9.6% and 4.2%, respectively. Moreover, the PEI-CDs-Cu2+ has excellent optical stability and good biocompatibility. Additionally, it is worth noting that the developed probe has successfully realized the visualization of GSH detection in MGC-803 cells.
2.
[Oxidative stress induced by NaAsO2 in HaCaT cells].
Sun, XC, Sun, GF, Liu, S, Zhang, Y
Wei sheng yan jiu = Journal of hygiene research. 2005;(1):46-8
Abstract
OBJECTIVE To study the level of oxidative stress induced by sodium arsenite (NaAsO2) in HaCaT cells. METHODS The AlamarBlue assay was used to evaluate the viability of HaCaT. The level of ROS was detected by staining. cells with DCFH-DA. The contents of reduced (GSH) and oxidated (GSSG) glutathione were detected with the fluorescent method. The apoptosis and necrosis rates were counted based on the PI staining. RESULTS The reduction of AlamarBlue increased in the cells treated at dose of 0.001 -1 micromol/L NaAsO2 and decreased in the cells treated at doses of over 10 micromol/L NaAsO2. The fluorescent density of DCF significantly increased in all experimental groups, the contents of GSH and GSSG increased in groups higher than that of 1 micromol/L and higher than that of 5 micromol/L respectively. The apoptosis and necrosis rates were increased markedly at dose of 20 micromol/L. CONCLUSION Arsenic could induce enhancive ROS in HaCaT. At low levels of arsenic the proliferation of HaCaT was stimulated while at high levels of arsenic was inhibited. The increase in contents of GSH and GSSG could be associated with the excessive ROS and with detoxification in HaCaT.