1.
[Progress in the plant GH3 gene family].
Sun, T, Chai, T, Liu, G, Zhang, Y
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2008;(11):1860-6
Abstract
Phytohormone auxins play important roles in plant growth and development. The primary auxin-response genes can be classified into three major groups: Aux/IAAs, SAURs and GH3s. Significant progress has been made in understanding these gene families by approaches of the functional genomics, molecular genetics and molecular biology. In this review, we focused on the structures, functions and models of the expressional regulation of plant GH3 genes. The interactions in the signal transduction pathways between auxins and other signals mediated by the GH3 genes, the relationship between the GH3 genes and the stress adaptation responses of plants are emphasized.
2.
Glucuronides are the main isoflavone metabolites in women.
Zhang, Y, Hendrich, S, Murphy, PA
The Journal of nutrition. 2003;(2):399-404
Abstract
Three experiments were conducted to characterize the metabolism of isoflavones from soy milk in women: two meals in 2 wk separated by a 1-wk washout period (Experiment 1), one meal feeding (Experiment 2) and six consecutive days of feeding (Experiment 3). Urine and plasma samples were extracted directly or predigested before extraction with H-2 beta-glucuronidase/sulfatase or B-3 beta-glucuronidase so that isoflavone glucuronide and sulfate conjugates could be determined by difference. Among the three experiments, no significant differences were found in the proportion of glucuronide, sulfate and aglycone isoflavones recovered from plasma samples taken 3 h after isoflavone dosing or in 24-h urine samples taken after isoflavone dosing. In the 6-d feeding study, samples taken on d 5 and 6 did not differ significantly in isoflavone content or proportion of the metabolites studied. The percentages of daidzein and genistein glucuronides were 73 +/- 4 and 71 +/- 5% of total daidzein and genistein excreted in urine, and 62 +/- 4 and 53 +/- 6% of total daidzein and genistein present in plasma, respectively. Percentages of aglycone daidzein and genistein were 4 +/- 1 and 5 +/- 1% of total daidzein and genistein in urine, and 18 +/- 2 and 26 +/- 7% of total daidzein and genistein present in plasma, respectively. These studies showed that about one fifth of circulating isoflavones are aglycones. Concentrations of isoflavones chosen for in vitro studies should take this into account. Because the glucuronide isoflavones predominate in vivo, these metabolites should not be overlooked as possible contributors to observed effects of isoflavones.