1.
Rice functional genomics: decades' efforts and roads ahead.
Chen, R, Deng, Y, Ding, Y, Guo, J, Qiu, J, Wang, B, Wang, C, Xie, Y, Zhang, Z, Chen, J, et al
Science China. Life sciences. 2022;(1):33-92
Abstract
Rice (Oryza sativa L.) is one of the most important crops in the world. Since the completion of rice reference genome sequences, tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks. In this review, we summarize the research progress of rice biology over past decades, including omics, genome-wide association study, phytohormone action, nutrient use, biotic and abiotic responses, photoperiodic flowering, and reproductive development (fertility and sterility). For the roads ahead, cutting-edge technologies such as new genomics methods, high-throughput phenotyping platforms, precise genome-editing tools, environmental microbiome optimization, and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice, and facilitate integrations of the knowledge for agricultural applications.
2.
Hormone Treatments in Studying Leaf Senescence.
Zhang, Z, Guo, Y
Methods in molecular biology (Clifton, N.J.). 2018;:125-132
Abstract
As the last stage of plant development, senescence can be regulated by a large number of signals such as aging, reproductive growth, nutrient availability, and stresses. Various plant hormones have been shown to be involved in regulating plant senescence. For example, ethylene, abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and strigolactones (SLs) promote senescence, whereas cytokinins (CKs) inhibit senescence. Different hormones regulate senescence via distinct pathways, while cross talks between signaling pathways exist. In senescence-related studies, treating plants with various hormones to alter senescence is a common practice. In this chapter, we summarize experimental procedures of treating detached Arabidopsis leaves with a number of senescence-regulating hormones including ABA, SLs, MeJA, SA peptide hormones.
3.
Transcriptional regulation of receptor-like protein genes by environmental stresses and hormones and their overexpression activities in Arabidopsis thaliana.
Wu, J, Liu, Z, Zhang, Z, Lv, Y, Yang, N, Zhang, G, Wu, M, Lv, S, Pan, L, Joosten, MH, et al
Journal of experimental botany. 2016;(11):3339-51
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Abstract
Receptor-like proteins (RLPs) have been implicated in multiple biological processes, including plant development and immunity to microbial infection. Fifty-seven AtRLP genes have been identified in Arabidopsis, whereas only a few have been functionally characterized. This is due to the lack of suitable physiological screening conditions and the high degree of functional redundancy among AtRLP genes. To overcome the functional redundancy and further understand the role of AtRLP genes, we studied the evolution of AtRLP genes and compiled a comprehensive profile of the transcriptional regulation of AtRLP genes upon exposure to a range of environmental stresses and different hormones. These results indicate that the majority of AtRLP genes are differentially expressed under various conditions that were tested, an observation that will help to select certain AtRLP genes involved in a specific biological process for further experimental studies to eventually dissect their function. A large number of AtRLP genes were found to respond to more than one treatment, suggesting that one single AtRLP gene may be involved in multiple physiological processes. In addition, we performed a genome-wide cloning of the AtRLP genes, and generated and characterized transgenic Arabidopsis plants overexpressing the individual AtRLP genes, presenting new insight into the roles of AtRLP genes, as exemplified by AtRLP3, AtRLP11 and AtRLP28 Our study provides an overview of biological processes in which AtRLP genes may be involved, and presents valuable resources for future investigations into the function of these genes.