1.
Advances in research on functional genes of tea plant.
Zhang, Z, Feng, X, Wang, Y, Xu, W, Huang, K, Hu, M, Zhang, C, Yuan, H
Gene. 2019;:143940
Abstract
Tea plant (Camellia sinensis) is an important leaf-type woody crop used to produce non-alcoholic beverages all over the world. Tea is one of the oldest and most popular non-alcoholic beverages in the world, and long-term tea drinking has numerous healthful for humans due to many of the important secondary metabolites, such as polyphenols and theanine. Theanine and polyphenols are also closely related to tea flavor and tea aroma, which is usually as the standard for judging tea quality. The growth of tea plants and quality of teas are susceptible to adversity abiotic and biotic stresses, such as low temperatures and pests. Consequently, this review focus on the research progress of key genes related to the stress resistance and material metabolism of tea plants in recent years. We aim at comprehensively understanding the growth and metabolism of tea plants and their relationship with the external environment, so as to provide an in-depth and broad theoretical support for the breeding of excellent tea plant varieties.
2.
Divergence in function and expression of the NOD26-like intrinsic proteins in plants.
Liu, Q, Wang, H, Zhang, Z, Wu, J, Feng, Y, Zhu, Z
BMC genomics. 2009;:313
Abstract
BACKGROUND NOD26-like intrinsic proteins (NIPs) that belong to the aquaporin superfamily are plant-specific and exhibit a similar three-dimensional structure. Experimental evidences however revealed that functional divergence should have extensively occurred among NIP genes. It is therefore intriguing to further investigate the evolutionary mechanisms being responsible for the functional diversification of the NIP genes. To better understand this process, a comprehensive analysis including the phylogenetic, positive selection, functional divergence, and transcriptional analysis was carried out. RESULTS The origination of NIPs could be dated back to the primitive land plants, and their diversification would be no younger than the emergence time of the moss P. patens. The rapid proliferation of NIPs in plants may be primarily attributed to the segmental chromosome duplication produced by polyploidy and tandem duplications. The maximum likelihood analysis revealed that NIPs should have experienced strong selective pressure for adaptive evolution after gene duplication and/or speciation, prompting the formation of distinct NIP groups. Functional divergence analysis at the amino acid level has provided strong statistical evidence for shifted evolutionary rate and/or radical change of the physiochemical properties of amino acids after gene duplication, and DIVERGE2 has identified the critical amino acid sites that are thought to be responsible for the divergence for further investigation. The expression of plant NIPs displays a distinct tissue-, cell-type-, and developmental specific pattern, and their responses to various stress treatments are quite different also. The differences in organization of cis-acting regulatory elements in the promoter regions may partially explain their distinction in expression. CONCLUSION A number of analyses both at the DNA and amino acid sequence levels have provided strong evidences that plant NIPs have suffered a high divergence in function and expression during evolution, which is primarily attributed to the strong positive selection or a rapid change of evolutionary rate and/or physiochemical properties of some critical amino acid sites.