1.
Effects of selenium on peripheral blood mononuclear cell membrane fluidity, interleukin-2 production and interleukin-2 receptor expression in patients with chronic hepatitis.
He, SX, Wu, B, Chang, XM, Li, HX, Qiao, W
World journal of gastroenterology. 2004;(23):3531-3
Abstract
AIM: To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immune function in patients with chronic hepatitis. METHODS PBMCs were pretreated with selenium (1.156x10(-7) mol/L) for 6 h in vitro or extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk (200 microg/d), and then exposed to Con-A for 48 h. The membrane fluidity, interleukin-2 (IL-2) production and interleukin-2 receptor (IL-2R) expression in PBMCs and malondialdehyde (MDA) concentration in medium and lipid peroxide (LPO) in plasma were determined. RESULTS The PBMC membrane fluidity, IL-2 production and IL-2R expression in patients with chronic hepatitis were significantly lower than those in healthy blood donators (particle adhesive degree R, 0.17+/-0.01 vs 0.14+/-0.01, P<0.01; IL-2, 40.26+/-9.55 vs 72.96+/-11.36, P<0.01; IL-2R, 31.05+/-5.09 vs 60.58+/-10.56, P<0.01), and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood donators (1.44+/-0.08 vs 0.93+/-0.08, P<0.01). Both in vitro and in vivo administration of selenium could reverse the above parameters. CONCLUSION Supplement of selenium can suppress lipid peroxidation, and improve PBMC membrane fluidity and immune function in patients with chronic hepatitis.
2.
[Correction of immune disorders with neoselenium in patients with chronic bronchitis].
Kalinina, EP, Zhuravskaia, NS, Tsyvkina, GI, Koziavina, NV
Klinicheskaia meditsina. 2003;(3):43-6
Abstract
The examination covered 55 patients with chronic bronchitis (mean age 58 +/- 4.2 years, mean duration of the disease 11.2 +/- 5.2 years). All the patients were in remission and received broncholytics. In 32 of them, basic treatment was combined with a course of immunorehabilitation with neoselenium. The effect of the latter was assessed at immunological examination of the patients before and after the treatment with indirect immunofluorescence with monoclonal antibodies. Neoselenium appeared to have an immunomodulating action on all immunity links, it enhanced functional activity and bactericidity of antigen-presenting cells and cytotoxic activity of natural killers, diminished the tension in the function of endogenic antioxidant systems.